English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Mic60 exhibits a coordinated clustered distribution along and across yeast and mammalian mitochondria.

Stoldt, S., Stephan, T., Jans, D. C., Brüser, C., Lange, F., Keller-Findeisen, J., et al. (2019). Mic60 exhibits a coordinated clustered distribution along and across yeast and mammalian mitochondria. Proceedings of the National Academy of Sciences of the United States of America, 116(20), 9853-9858. doi:10.1073/pnas.1820364116.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-7F2B-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-9BD5-E
Genre: Journal Article

Files

show Files
hide Files
:
3052463.pdf (Publisher version), 2MB
Name:
3052463.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3052463_Suppl.pdf (Supplementary material), 5MB
Name:
3052463_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Stoldt, S.1, Author              
Stephan, T.1, Author              
Jans, D. C., Author
Brüser, C.1, Author              
Lange, F.1, Author              
Keller-Findeisen, J.2, Author              
Riedel, D.3, Author              
Hell, S. W.2, Author              
Jakobs, S.1, Author              
Affiliations:
1Research Group of Mitochondrial Structure and Dynamics, MPI for Biophysical Chemistry, Max Planck Society, ou_578566              
2Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
3Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

Content

show
hide
Free keywords: FIB-SEM; MICOS; mitochondria; mitoskeleton; nanoscopy
 Abstract: Mitochondria are tubular double-membrane organelles essential for eukaryotic life. They form extended networks and exhibit an intricate inner membrane architecture. The MICOS (mitochondrial contact site and cristae organizing system) complex, crucial for proper architecture of the mitochondrial inner membrane, is localized primarily at crista junctions. Harnessing superresolution fluorescence microscopy, we demonstrate that Mic60, a subunit of the MICOS complex, as well as several of its interaction partners are arranged into intricate patterns in human and yeast mitochondria, suggesting an ordered distribution of the crista junctions. We show that Mic60 forms clusters that are preferentially localized in the inner membrane at two opposing sides of the mitochondrial tubules so that they form extended opposing distribution bands. These Mic60 distribution bands can be twisted, resulting in a helical arrangement. Focused ion beam milling-scanning electron microscopy showed that in yeast the twisting of the opposing distribution bands is echoed by the folding of the inner membrane. We show that establishment of the Mic60 distribution bands is largely independent of the cristae morphology. We suggest that Mic60 is part of an extended multiprotein interaction network that scaffolds mitochondria.

Details

show
hide
Language(s): eng - English
 Dates: 2019-04-262019-05-14
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1820364116
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 116 (20) Sequence Number: - Start / End Page: 9853 - 9858 Identifier: -