English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Engineered SUMO/protease system identifies Pdr6 as a bidirectional nuclear transport receptor.

Vera Rodriguez, A., Frey, S., & Görlich, D. (2019). Engineered SUMO/protease system identifies Pdr6 as a bidirectional nuclear transport receptor. The Journal of Cell Biology, 218(6), 2006-2020. doi:10.1083/jcb.201812091.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-7F59-C Version Permalink: http://hdl.handle.net/21.11116/0000-0003-CAC9-7
Genre: Journal Article

Files

show Files
hide Files
:
3052485.pdf (Publisher version), 3MB
Name:
3052485.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Vera Rodriguez, A.1, Author              
Frey, S.1, Author              
Görlich, D.1, Author              
Affiliations:
1Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society, ou_578574              

Content

show
hide
Free keywords: -
 Abstract: Cleavage of affinity tags by specific proteases can be exploited for highly selective affinity chromatography. The SUMO/SENP1 system is the most efficient for such application but fails in eukaryotic expression because it cross-reacts with endogenous proteases. Using a novel selection system, we have evolved the SUMOEu/SENP1Eu pair to orthogonality with the yeast and animal enzymes. SUMOEu fusions therefore remain stable in eukaryotic cells. Likewise, overexpressing a SENP1Eu protease is nontoxic in yeast. We have used the SUMOEu system in an affinity-capture-proteolytic-release approach to identify interactors of the yeast importin Pdr6/Kap122. This revealed not only further nuclear import substrates such as Ubc9, but also Pil1, Lsp1, eIF5A, and eEF2 as RanGTP-dependent binders and thus as export cargoes. We confirmed that Pdr6 functions as an exportin in vivo and depletes eIF5A and eEF2 from cell nuclei. Thus, Pdr6 is a bidirectional nuclear transport receptor (i.e., a biportin) that shuttles distinct sets of cargoes in opposite directions.

Details

show
hide
Language(s): eng - English
 Dates: 2019-04-252019-06-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1083/jcb.201812091
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Cell Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 218 (6) Sequence Number: - Start / End Page: 2006 - 2020 Identifier: -