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  BRACHYURY directs histone acetylation to target loci during mesoderm development

Beisaw, A., Tsaytler, P., Koch, F., Schmitz, S. U., Melissari, M.-T., Senf, A. D., et al. (2018). BRACHYURY directs histone acetylation to target loci during mesoderm development. EMBO Reports, 19(1), 118-134. doi:10.15252/embr.201744201.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-855F-D Version Permalink: http://hdl.handle.net/21.11116/0000-0003-8560-A
Genre: Journal Article

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 Creators:
Beisaw, Arica , Author
Tsaytler, Pavel , Author
Koch, Frederic , Author
Schmitz, Sandra U. , Author
Melissari, Maria-Theodora , Author
Senf, Anna D. , Author
Wittler, Lars1, Author              
Pennimpede, Tracie , Author
Macura, Karol1, Author              
Herrmann, Bernhard G.1, Author              
Grote, Phillip , Author
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

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Free keywords: Brachyury ; Lmo2 ; H3K27 acetylation; T‐box factors; autoregulation
 Abstract: T-box transcription factors play essential roles in multiple aspects of vertebrate development. Here, we show that cooperative function of BRACHYURY (T) with histone-modifying enzymes is essential for mouse embryogenesis. A single point mutation (TY88A) results in decreased histone 3 lysine 27 acetylation (H3K27ac) at T target sites, including the T locus, suggesting that T autoregulates the maintenance of its expression and functions by recruiting permissive chromatin modifications to putative enhancers during mesoderm specification. Our data indicate that T mediates H3K27ac recruitment through a physical interaction with p300. In addition, we determine that T plays a prominent role in the specification of hematopoietic and endothelial cell types. Hematopoietic and endothelial gene expression programs are disrupted in TY88A mutant embryos, leading to a defect in the differentiation of hematopoietic progenitors. We show that this role of T is mediated, at least in part, through activation of a distal Lmo2 enhancer.

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Language(s): eng - English
 Dates: 2017-10-252017-11-152018-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.15252/embr.201744201
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Title: EMBO Reports
  Other : EMBO Rep.
Source Genre: Journal
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Publ. Info: Oxford, UK : Published for EMBO by Oxford University Press
Pages: 17 Volume / Issue: 19 (1) Sequence Number: - Start / End Page: 118 - 134 Identifier: ISSN: 1469-221X
CoNE: https://pure.mpg.de/cone/journals/resource/110978984569661