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  Two-photon Bessel beam tomography for fast volume imaging

Seelig, J. D., & Flores Valle, A. (2019). Two-photon Bessel beam tomography for fast volume imaging. Optics Express, 27(9), 12147-12162. doi:doi.org/10.1364/OE.27.012147.

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2019_Seelig.pdf (Publisher version), 23MB
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Seelig, Johannes Dominik1, Author              
Flores Valle, Andres1, Author              
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1Max Planck Research Group Neural Circuits, Center of Advanced European Studies and Research (caesar), Max Planck Society, ou_2237639              

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 Abstract: Light microscopy on dynamic samples, for example neural activity in the brain, often requires imaging volumes that extend over several 100 µm in axial direction at a rate of at least several tens of Hertz. Here, we develop a tomography approach for scanning fluorescence microscopy which allows recording a volume image in a single frame scan. Volumes are imaged by simultaneously recording four independent projections at different angles using temporally multiplexed, tilted Bessel beams. From the resulting projections, three-dimensional images are reconstructed using inverse Radon transforms combined with convolutional neural networks (U-net).

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Language(s): eng - English
 Dates: 2019-04-17
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: doi.org/10.1364/OE.27.012147
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Title: Optics Express
  Abbreviation : Opt. Express
Source Genre: Journal
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Publ. Info: Washington, DC : Optical Society of America
Pages: - Volume / Issue: 27 (9) Sequence Number: - Start / End Page: 12147 - 12162 Identifier: ISSN: 1094-4087
CoNE: https://pure.mpg.de/cone/journals/resource/954925609918