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  Detection of single action potentials in vitro and in vivo with genetically-encoded Ca2+ sensors

Meyer zum Alten Borgloh, S., Haydon-Wallace, D., Astori, S., Yang, Y., Bausen, M., Kugler, S., et al. (2008). Detection of single action potentials in vitro and in vivo with genetically-encoded Ca2+ sensors. Poster presented at 38th Annual Meeting of the Society for Neuroscience (Neuroscience 2008), Washington, DC, USA.

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Meyer zum Alten Borgloh, S, Author
Haydon-Wallace, DJ, Author
Astori, S, Author
Yang, Y, Author
Bausen, M, Author
Kugler, S, Author
Mank, M, Author
Griesbeck, =, Author
Nakai, J, Author
Miyawaki, A, Author
Palmer, AE, Author
Tsien, RY, Author
Sprengel, R, Author
Kerr, JND1, 2, 3, Author           
Denk, W, Author
Hasan, MT, Author
Affiliations:
1Former Research Group Network Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_2528697              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              
3Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497807              

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 Abstract: Measurement of population activity with single-neuron resolution is pivotal for understanding how information is represented and processed in the brain and how the brain's responses are altered by experience. Because neuronal activity in the neocortex is sparse and different neuron types perform different tasks, such measurements need to resolve single action potentials in single neurons, and need to be targeted to neuronal sub-classes. This is greatly facilitated by the use of genetically-encoded fluorescent calcium indicator proteins (FCIPs) of neuronal activity. We have employed recombinant adeno-associated viruses to deliver different FCIPs to neurons at a sufficiently high levels to detect the Ca2+ transients that accompany single action potentials. Based on these transients we were able to detect action potentials with high reliability not only in cultured brain slices but also in cortical layer 2/3 pyramidal cells in living animals. Cell-type targeting and long-term recording thus make FCIPs highly suitable to follow the activity of identified cells over the periods of weeks to months. This allows the study of the development and plasticity of neural maps. Preliminary results suggest that with FCIPs functional imaging of the same cells is possible over periods of

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Language(s): eng - English
 Dates: 2008-11
 Publication Status: Published in print
 Pages: 1
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 Table of Contents: -
 Rev. Type: Peer
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Title: 38th Annual Meeting of the Society for Neuroscience (Neuroscience 2008)
Place of Event: Washington, DC, USA
Start-/End Date: 2008-11-15 - 2008-11-19

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Title: 38th Annual Meeting of the Society for Neuroscience (Neuroscience 2008)
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: 496.7 Start / End Page: - Identifier: -