English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Robust smartphone assisted biosensing based on asymmetric nanofluidic grating interferometry.

Purr, F., Eckardt, M. F., Kieserling, J., Gronwald, P. L., Burg, T. P., & Dietzel, A. (2019). Robust smartphone assisted biosensing based on asymmetric nanofluidic grating interferometry. Sensors, 19(9): 2065. doi:10.3390/s19092065.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-8D90-B Version Permalink: http://hdl.handle.net/21.11116/0000-0003-8D94-7
Genre: Journal Article

Files

show Files
hide Files
:
3053713.pdf (Publisher version), 3MB
Name:
3053713.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
3053713_Suppl.htm (Supplementary material), 265KB
Name:
3053713_Suppl.htm
Description:
-
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Purr, F.1, Author              
Eckardt, M. F., Author
Kieserling, J., Author
Gronwald, P. L., Author
Burg, T. P.1, Author              
Dietzel, A., Author
Affiliations:
1Research Group of Biological Micro- and Nanotechnology, MPI for Biophysical Chemistry, Max Planck Society, ou_578602              

Content

show
hide
Free keywords: biosensing; common mode rejection; interferometry; nanofluidic; optofluidic grating; portable point-of-care (POC); smartphone-based
 Abstract: Point-of-care systems enable fast therapy decisions on site without the need of any healthcare infrastructure. In addition to the sensitive detection, stable measurement by inexperienced persons outside of laboratory facilities is indispensable. A particular challenge in field applications is to reduce interference from environmental factors, such as temperature, to acceptable levels without sacrificing simplicity. Here, we present a smartphone-based point-of-care sensor. The method uses an optofluidic grating composed of alternating detection and reference channels arranged as a reflective phase grating. Biomolecules adsorbing to the detection channel alter the optical path length, while the parallel reference channels enable a direct common mode rejection within a single measurement. The optical setup is integrated in a compact design of a mobile readout device and the usability is ensured by a smartphone application. Our results show that different ambient temperatures do not have any influence on the signal. In a proof-of concept experiment we measured the accumulation of specific molecules in functionalized detection channels in real-time and without the need of any labeling. Therefore, the channel walls have been modified with biotin as capture molecules and the specific binding of streptavidin was detected. A mobile, reliable and robust point-of-care device has been realized by combining an inherently differential measurement concept with a smartphone-based, mobile readout device.

Details

show
hide
Language(s): eng - English
 Dates: 2019-05-032019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.3390/s19092065
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Sensors
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 14 Volume / Issue: 19 (9) Sequence Number: 2065 Start / End Page: - Identifier: -