English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Assessment of Established Techniques to Determine Developmental and Malignant Potential of Human Pluripotent Stem Cells

Allison, T. F., Andrews, P. W., Avior, Y., Barbaric, I., Benvenisty, N., Bock, C., et al. (2018). Assessment of Established Techniques to Determine Developmental and Malignant Potential of Human Pluripotent Stem Cells. Nature Communications, 9(1): 1925. doi:10.1038/s41467-018-04011-3.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Allison.pdf (Publisher version), 3MB
Name:
Allison.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© The Author(s) 2018

Locators

show

Creators

show
hide
 Creators:
Allison, Thomas F., Author
Andrews, Peter W., Author
Avior, Yishai, Author
Barbaric, Ivana, Author
Benvenisty, Nissim, Author
Bock, Christoph, Author
Brehm, Jennifer, Author
Bruestle, Oliver, Author
Damjanov, Ivan, Author
Elefanty, Andrew, Author
Felkner, Daniel, Author
Gokhale, Paul J., Author
Halbritter, Florian, Author
Healy, Lyn E., Author
Hu, Tim X., Author
Knowles, Barbara B., Author
Loring, Jeanne F., Author
Ludwig, Tenneille E., Author
Mayberry, Robyn, Author
Micallef, Suzanne, Author
Mohamed, Jameelah S., AuthorMüller, Franz-Josef1, Author           Mummery, Christine L., AuthorNakatsuji, Norio, AuthorNg, Elizabeth S., AuthorOh, Steve K. W., AuthorO'Shea, Orla, AuthorPera, Martin F., AuthorReubinoff, Benjamin, AuthorRobson, Paul, AuthorRossant, Janet, AuthorSchuldt, Bernhard M., AuthorSolter, Davor, AuthorSourris, Koula, AuthorStacey, Glyn, AuthorStanley, Edouard G., AuthorSuemori, Hirofumi, AuthorTakahashi, Kazutoshi, AuthorYamanaka, Shinya, Author more..
Affiliations:
1Cellular Phenotyping (Franz-Josef Müller), Dept. of Genome Regulation, (Head: Alexander Meissner), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_3014190              

Content

show
hide
Free keywords: -
 Abstract: The International Stem Cell Initiative compared several commonly used approaches to assess human pluripotent stem cells (PSC). PluriTest predicts pluripotency through bioinformatic analysis of the transcriptomes of undifferentiated cells, whereas, embryoid body (EB) formation in vitro and teratoma formation in vivo provide direct tests of differentiation. Here we report that EB assays, analyzed after differentiation under neutral conditions and under conditions promoting differentiation to ectoderm, mesoderm, or endoderm lineages, are sufficient to assess the differentiation potential of PSCs. However, teratoma analysis by histologic examination and by TeratoScore, which estimates differential gene expression in each tumor, not only measures differentiation but also allows insight into a PSC's malignant potential. Each of the assays can be used to predict pluripotent differentiation potential but, at this stage of assay development, only the teratoma assay provides an assessment of pluripotency and malignant potential, which are both relevant to the pre-clinical safety assessment of PSCs.

Details

show
hide
Language(s): eng - English
 Dates: 2018-03-262018-05-15
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: BibTex Citekey: Allison2018
DOI: 10.1038/s41467-018-04011-3
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: 15 Volume / Issue: 9 (1) Sequence Number: 1925 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723