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  Benchmarking miniaturized microscopy against two-photon calcium imaging using single-cell orientation tuning in mouse visual cortex

Glas, A., Hübener, M., Bonhoeffer, T., & Goltstein, P. M. (2019). Benchmarking miniaturized microscopy against two-photon calcium imaging using single-cell orientation tuning in mouse visual cortex. PLoS One, 14(4): e0214954. doi:10.1371/journal.pone.0214954.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-8EA9-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-8EAA-E
Genre: Journal Article

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journal.pone.0214954.pdf (Publisher version), 5MB
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© 2019 Glas et al.

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 Creators:
Glas, Annet1, Author              
Hübener, Mark1, Author              
Bonhoeffer, Tobias1, Author              
Goltstein, Pieter M.1, Author              
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1Department: Synapses-Circuits-Plasticity / Bonhoeffer, MPI of Neurobiology, Max Planck Society, ou_1113545              

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Free keywords: RECEPTIVE-FIELDS; SUBTYPES; NEURONSScience & Technology - Other Topics;
 Abstract: Miniaturized microscopes are lightweight imaging devices that allow optical recordings from neurons in freely moving animals over the course of weeks. Despite their ubiquitous use, individual neuronal responses measured with these microscopes have not been directly compared to those obtained with established in vivo imaging techniques such as bench-top two-photon microscopes. To achieve this, we performed calcium imaging in mouse primary visual cortex while presenting animals with drifting gratings. We identified the same neurons in image stacks acquired with both microscopy methods and quantified orientation tuning of individual neurons. The response amplitude and signal-to-noise ratio of calcium transients recorded upon visual stimulation were highly correlated between both microscopy methods, although influenced by neuropil contamination in miniaturized microscopy. Tuning properties, calculated for individual orientation tuned neurons, were strongly correlated between imaging techniques. Thus, neuronal tuning features measured with a miniaturized microscope are quantitatively similar to those obtained with a two-photon microscope.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 18
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 14 (4) Sequence Number: e0214954 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850