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  Chronic dysfunction of Stromal interaction molecule by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila.

Xu, Y., Borcherding, A. F., Heier, C., Tian, G., Roeder, T., & Kühnlein, R. P. (2019). Chronic dysfunction of Stromal interaction molecule by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila. Scientific Reports, 9: 6989. doi:10.1038/s41598-019-43327-y.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-925F-E Version Permalink: http://hdl.handle.net/21.11116/0000-0003-9266-5
Genre: Journal Article

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 Creators:
Xu, Y.1, Author              
Borcherding, A. F., Author
Heier, C., Author
Tian, G., Author
Roeder, T., Author
Kühnlein, R. P.1, Author              
Affiliations:
1Research Group of Molecular Physiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578592              

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 Abstract: Obesity is a progressive, chronic disease, which can be caused by long-term miscommunication between organs. It remains challenging to understand how chronic dysfunction in a particular tissue remotely impairs other organs to eventually imbalance organismal energy homeostasis. Here we introduce RNAi Pulse Induction (RiPI) mediated by short hairpin RNA (shRiPI) or double-stranded RNA (dsRiPI) to generate chronic, organ-specific gene knockdown in the adult Drosophila fat tissue. We show that organ-restricted RiPI targeting Stromal interaction molecule (Stim), an essential factor of store-operated calcium entry (SOCE), results in progressive fat accumulation in fly adipose tissue. Chronic SOCE-dependent adipose tissue dysfunction manifests in considerable changes of the fat cell transcriptome profile, and in resistance to the glucagon-like Adipokinetic hormone (Akh) signaling. Remotely, the adipose tissue dysfunction promotes hyperphagia likely via increased secretion of Akh from the neuroendocrine system. Collectively, our study presents a novel in vivo paradigm in the fly, which is widely applicable to model and functionally analyze inter-organ communication processes in chronic diseases.

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Language(s): eng - English
 Dates: 2019-05-06
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41598-019-43327-y
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Title: Scientific Reports
Source Genre: Journal
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Pages: 15 Volume / Issue: 9 Sequence Number: 6989 Start / End Page: - Identifier: -