English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A cytochrome P450 from juvenile mustard leaf beetles hydroxylates geraniol, a key step in iridoid biosynthesis

Fu, N., Yang, Z.-l., Pauchet, Y., Paetz, C., Brandt, W., Boland, W., et al. (2019). A cytochrome P450 from juvenile mustard leaf beetles hydroxylates geraniol, a key step in iridoid biosynthesis. Insect Biochemistry and Molecular Biology, 113: 103212. doi:10.1016/j.ibmb.2019.103212.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
BOL713.pdf (Publisher version), 3MB
 
File Permalink:
-
Name:
BOL713.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
BOL713s1.zip (Supplementary material), 216KB
 
File Permalink:
-
Name:
BOL713s1.zip
Description:
-
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/zip
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Fu, Nanxia1, 2, Author              
Yang, Zhi-ling3, Author              
Pauchet, Yannick4, Author              
Paetz, Christian5, Author              
Brandt, Wolfgang, Author
Boland, Wilhelm6, Author              
Burse, Antje6, Author              
Affiliations:
1Research Group Dr. A. Burse, Chemical Defense of Leaf Beetles, Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_543545              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
3Research Group Dr. F. Beran, Detoxification in Insects, MPI for Chemical Ecology, Max Planck Society, ou_1933291              
4Department of Entomology, Prof. D. G. Heckel, MPI for Chemical Ecology, Max Planck Society, ou_421895              
5Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society, ou_421898              
6Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              

Content

show
hide
Free keywords: -
 Abstract: Juveniles of the leaf beetle Phaedon cochleariae synthesize iridoid via the mevalonate pathway to repel predators. The normal terpenoid biosynthesis is integrated into the dedicated defensive pathway by the ω-hydroxylation of geraniol to 8-hydroxygeraniol. Here we identify and characterize the geraniol 8-hydroxylase as a P450 monooxygenase using integrated transcriptomic and proteomic analyses. In the fat body, 73 individual cytochrome P450s were identified. The double stranded RNA (dsRNA)-mediated knock down of CYP6BH5 led to a significant reduction of 8-hydroxygeraniol-glucoside in the hemolymph and, later, of the chrysomelidial in the defensive secretion. Heterologously expressed CYP6BH5 converted geraniol to 8-hydroxygeraniol. In addition to geraniol, CYP6BH5 also catalyzes other monoterpenols, such as nerol and citronellol, into the corresponding α, ω-dihydroxy compounds.

Details

show
hide
Language(s):
 Dates: 2019-08-162019-10
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Insect Biochemistry and Molecular Biology
  Other : Insect Biochem. Mol. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford [England] : Pergamon
Pages: - Volume / Issue: 113 Sequence Number: 103212 Start / End Page: - Identifier: ISSN: 0965-1748
CoNE: https://pure.mpg.de/cone/journals/resource/954925581163