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  The Ca2+ channel CNGC19 regulates Arabidopsis defense against Spodoptera herbivory

Meena, M. K., Prajapati, R., Krishna, D., Divakaran, K., Pandey, Y., Reichelt, M., et al. (2019). The Ca2+ channel CNGC19 regulates Arabidopsis defense against Spodoptera herbivory. The Plant Cell, 31(7), 1539-1562. doi:10.1105/tpc.19.00057.

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 Creators:
Meena, Mukesh Kumar, Author
Prajapati, Ramgopal, Author
Krishna, Deepthi, Author
Divakaran, Keerthi, Author
Pandey, Yogesh, Author
Reichelt, Michael1, Author              
Mathew, MK, Author
Boland, Wilhelm2, Author              
Mithöfer, Axel3, Author              
Vadassery, Jyothilakshmi2, Author              
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
2Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              
3Research Group Plant Defense Physiology, Dr. Axel Mithöfer, MPI for Chemical Ecology, Max Planck Society, ou_3022756              

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 Abstract: Cellular calcium elevation is an important signal used by plants for recognition and signaling of environmental stress. Perception of the generalist insect, Spodoptera litura, by Arabidopsis thaliana activates cytosolic Ca2+ elevation, which triggers downstream defense. However, not all the Ca2+ channels generating the signal have been identified, nor are their modes of action known. We report on a rapidly activated, leaf vasculature- and plasma membrane-localized, CYCLIC NUCLEOTIDE GATED CHANNEL19 (CNGC19), which activates herbivory-induced Ca2+ flux and plant defense. Loss of CNGC19 function results in decreased herbivory defense. The cngc19 mutant shows aberrant and attenuated intra-vascular Ca2+ fluxes. CNGC19 is a Ca2+ permeable channel, as hyperpolarization of CNGC19-expressing Xenopus oocytes in the presence of both cAMP and Ca2+ results in Ca2+ influx. Breakdown of Ca2+-based defence in cngc19 mutants leads to a decrease in herbivory-induced JA-Ile biosynthesis and expression of JA responsive genes. cngc19 mutants are deficient in aliphatic glucosinolate accumulation and hyperaccumulate its precursor, methionine. CNGC19 modulates aliphatic glucosinolate biosynthesis in tandem with BRANCHED-CHAIN AMINO ACID TRANSAMINASE4 (BCAT4), which is involved in the chain elongation pathway of Met-derived glucosinolates. Furthermore, CNGC19 interacts with herbivory-induced CALMODULIN2 (CaM2) in planta. Together, our work reveals a key mechanistic role for the Ca2+ channel CNGC19 in the recognition of herbivory and the activation of defense signaling.

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 Dates: 2019-05-072019-072019-07
 Publication Status: Published in print
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 Identifiers: Other: MIT007
DOI: 10.1105/tpc.19.00057
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 31 (7) Sequence Number: - Start / End Page: 1539 - 1562 Identifier: ISSN: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X