English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  retroLEAP: rAAV-based retrograde trans-synaptic labeling, expression and perturbation

Reinert, J. K., Sonntag, I., Sonntag, H., Sprengel, R., Pelzer, P., Lessle, S., et al. (2019). retroLEAP: rAAV-based retrograde trans-synaptic labeling, expression and perturbation. Nature methods, e-pup: 928, pp. 1-13. doi:10.1101/537928.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-A0FF-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-A103-3
Genre: Journal Article

Files

show Files
hide Files
:
bioRxiv_epub_2019_537928.pdf (Any fulltext), 13MB
 
File Permalink:
-
Name:
bioRxiv_epub_2019_537928.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, Heidelberg; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Locator:
https://doi.org/10.1101/537928 (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Reinert, Janine K, Author
Sonntag, Ivo, Author
Sonntag, Hannah1, Author              
Sprengel, Rolf2, 3, 4, Author              
Pelzer, Patric, Author
Lessle, Sascha, Author
Kaiser, Michaela, Author
Kuner, Thomas, Author
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
3Olfaction Web, Max Planck Institute for Medical Research, Max Planck Society, ou_1497733              
4Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497741              

Content

show
hide
Free keywords: -
 Abstract: Identifying and manipulating synaptically connected neurons across brain regions remains a core challenge in understanding complex nervous systems. RetroLEAP is a novel approach for retrograde trans-synaptic Labelling, Expression And Perturbation. GFP-dependent recombinase (Flp-DOG) detects trans-synaptic transfer of GFP-tetanus toxin heavy chain fusion protein (GFP-TTC) and activates expression of any gene of interest in synaptically connected cells. RetroLEAP overcomes existing limitations, is non-toxic, highly flexible, efficient, sensitive and easy to implement.

Details

show
hide
Language(s): eng - English
 Dates: 2019-02-01
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1101/537928
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature methods
  Other : Nature methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: e-pup Sequence Number: 928 Start / End Page: 1 - 13 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556