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  PLK4 is a microtubule-associated protein that self-assembles promoting de novo MTOC formation.

Gouveia, S. M., Zitouni, S., Kong, D., Duarte, P., Gomes, B. F., Sousa, A. L., et al. (2018). PLK4 is a microtubule-associated protein that self-assembles promoting de novo MTOC formation. Journal of cell science, 132(4): jcs219501. doi:10.1242/jcs.219501.

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 Creators:
Gouveia, Susana Montenegro, Author
Zitouni, Sihem, Author
Kong, Dong, Author
Duarte, Paulo, Author
Gomes, Beatriz Ferreira1, Author           
Sousa, Ana Laura, Author
Tranfield, Erin M, Author
Hyman, Anthony1, Author           
Loncarek, Jadranka, Author
Bettencourt-Dias, Monica, Author
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

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 Abstract: The centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4, is critical for the recruitment of several centriole components. Here, we investigate the beginning of centrosome biogenesis, taking advantage of Xenopus egg extracts, where PLK4 can induce de novo MTOC formation ( Eckerdt et al., 2011; Zitouni et al., 2016). Surprisingly, we observe that in vitro, PLK4 can self-assemble into condensates that recruit α- and β-tubulins. In Xenopus extracts, PLK4 assemblies additionally recruit STIL, a substrate of PLK4, and the microtubule nucleator γ-tubulin, forming acentriolar MTOCs de novo The assembly of these robust microtubule asters is independent of dynein, similar to what is found for centrosomes. We suggest a new mechanism of action for PLK4, where it forms a self-organising catalytic scaffold that recruits centriole components, PCM factors and α- and β-tubulins, leading to MTOC formation.This article has an associated First Person interview with the first author of the paper.

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 Dates: 2018-11-09
 Publication Status: Issued
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 Identifiers: DOI: 10.1242/jcs.219501
Other: cbg-7238
PMID: 30237222
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Title: Journal of cell science
  Other : J Cell Sci
Source Genre: Journal
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Pages: - Volume / Issue: 132 (4) Sequence Number: jcs219501 Start / End Page: - Identifier: -