English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability.

Klemm, A., Bosilj, A., Gluncˇic, M., Pavin, N., & Tolic, I. M. (2018). Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability. Molecular biology of the cell, 29(11), 1332-1345. doi:10.1091/mbc.E17-11-0667.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Klemm, Anna1, Author           
Bosilj, Agneza, Author
Gluncˇic, Matko, Author
Pavin, Nenad1, Author           
Tolic, Iva M.1, Author           
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

Content

show
hide
Free keywords: -
 Abstract: During metaphase, sister chromatids are connected to microtubules extending from the opposite spindle poles via kinetochores to protein complexes on the chromosome. Kinetochores congress to the equatorial plane of the spindle and oscillate around it, with kinesin-8 motors restricting these movements. Yet, the physical mechanism underlying kinetochore movements is unclear. We show that kinetochore movements in the fission yeast Schizosaccharomyces pombe are regulated by kinesin-8-promoted microtubule catastrophe, force-induced rescue, and microtubule dynamic instability. A candidate screen showed that among the selected motors only kinesin-8 motors Klp5/Klp6 are required for kinetochore centering. Kinesin-8 accumulates at the end of microtubules, where it promotes catastrophe. Laser ablation of the spindle resulted in kinetochore movement toward the intact spindle pole in wild-type and klp5Δ cells, suggesting that kinetochore movement is driven by pulling forces. Our theoretical model with Langevin description of microtubule dynamic instability shows that kinesin-8 motors are required for kinetochore centering, whereas sensitivity of rescue to force is necessary for the generation of oscillations. We found that irregular kinetochore movements occur for a broader range of parameters than regular oscillations. Thus, our work provides an explanation for how regulation of microtubule dynamic instability contributes to kinetochore congression and the accompanying movements around the spindle center.

Details

show
hide
Language(s):
 Dates: 2018-06-01
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1091/mbc.E17-11-0667
Other: cbg-7165
PMID: 29851559
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular biology of the cell
  Other : Mol Biol Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 29 (11) Sequence Number: - Start / End Page: 1332 - 1345 Identifier: -