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  Genetic mapping of species differences via in vitro crosses in mouse embryonic stem cells.

Lazzarano, S., Kučka, M., Castro, J. P. L., Naumann, R., Medina, P., Fletcher, M. N. C., et al. (2018). Genetic mapping of species differences via in vitro crosses in mouse embryonic stem cells. Proceedings of the National Academy of Sciences of the United States of America, 115(14), 3680-3685. doi:10.1073/pnas.1717474115.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-F62C-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-F62D-6
Genre: Journal Article

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 Creators:
Lazzarano, Stefano, Author
Kučka, Marek, Author
Castro, João P L, Author
Naumann, Ronald1, Author              
Medina, Paloma, Author
Fletcher, Michael N C, Author
Wombacher, Rebecka, Author
Gribnau, Joost, Author
Hochepied, Tino, Author
Montagu, Marc Van, Author
Libert, Claude, Author
Chan, Yingguang Frank, Author
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

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 Abstract: Discovering the genetic changes underlying species differences is a central goal in evolutionary genetics. However, hybrid crosses between species in mammals often suffer from hybrid sterility, greatly complicating genetic mapping of trait variation across species. Here, we describe a simple, robust, and transgene-free technique to generate "in vitro crosses" in hybrid mouse embryonic stem (ES) cells by inducing random mitotic cross-overs with the drug ML216, which inhibits the DNA helicase Bloom syndrome (BLM). Starting with an interspecific F1 hybrid ES cell line between theMus musculuslaboratory mouse andMus spretus(∼1.5 million years of divergence), we mapped the genetic basis of drug resistance to the antimetabolite tioguanine to a single region containing hypoxanthine-guanine phosphoribosyltransferase (Hprt) in as few as 21 d through "flow mapping" by coupling in vitro crosses with fluorescence-activated cell sorting (FACS). We also show how our platform can enable direct study of developmental variation by rederiving embryos with contribution from the recombinant ES cell lines. We demonstrate how in vitro crosses can overcome major bottlenecks in mouse complex trait genetics and address fundamental questions in evolutionary biology that are otherwise intractable through traditional breeding due to high cost, small litter sizes, and/or hybrid sterility. In doing so, we describe an experimental platform toward studying evolutionary systems biology in mouse and potentially in human and other mammals, including cross-species hybrids.

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 Dates: 2018-04-03
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: DOI: 10.1073/pnas.1717474115
Other: cbg-7092
PMID: 29563231
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc Natl Acad Sci U.S.A.
Source Genre: Journal
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Pages: - Volume / Issue: 115 (14) Sequence Number: - Start / End Page: 3680 - 3685 Identifier: -