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  Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules.

Lüdecke, A., Seidel, A.-M., Braun, M., Lansky, Z., & Diez, S. (2018). Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules. Nature communications, 9(1): 2214. doi:10.1038/s41467-018-04656-0.

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 Creators:
Lüdecke, Annemarie1, Author           
Seidel, Anja-Maria, Author
Braun, Marcus1, Author           
Lansky, Zdenek1, Author           
Diez, Stefan1, Author           
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

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 Abstract: Form and function of the mitotic spindle depend on motor proteins that crosslink microtubules and move them relative to each other. Among these are kinesin-14s, such as Ncd, which interact with one microtubule via their non-processive motor domains and with another via their diffusive tail domains, the latter allowing the protein to slip along the microtubule surface. Little is known about the influence of the tail domains on the protein's performance. Here, we show that diffusive anchorage of Ncd's tail domains impacts velocity and force considerably. Tail domain slippage reduced velocities from 270 nm s-1 to 60 nm s-1 and forces from several piconewtons to the sub-piconewton range. These findings challenge the notion that kinesin-14 may act as an antagonizer of other crosslinking motors, such as kinesin-5, during mitosis. It rather suggests a role of kinesin-14 as a flexible element, pliantly sliding and crosslinking microtubules to facilitate remodeling of the mitotic spindle.

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 Dates: 2018-06-07
 Publication Status: Issued
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 Identifiers: DOI: 10.1038/s41467-018-04656-0
Other: cbg-7164
PMID: 29880831
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Title: Nature communications
  Other : Nat Commun
Source Genre: Journal
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Pages: - Volume / Issue: 9 (1) Sequence Number: 2214 Start / End Page: - Identifier: -