English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Genetic mechanism underlying sexual plasticity and its association with colour patterning in zebrafish (Danio rerio)

Hosseini, S., Ha, N. T., Simianer, H., Falker-Gieske, C., Brenig, B., Franke, A., et al. (2019). Genetic mechanism underlying sexual plasticity and its association with colour patterning in zebrafish (Danio rerio). BMC Genomics, 20: 341. doi:10.1186/s12864-019-5722-1.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-AEDC-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-AEDD-1
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hosseini, S., Author
Ha, N. T., Author
Simianer, H., Author
Falker-Gieske, C., Author
Brenig, B., Author
Franke, A., Author
Horstgen-Schwark, G., Author
Tetens, J., Author
Herzog, Sebastian1, Author              
Sharifi, A. R., Author
Affiliations:
1Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              

Content

show
hide
Free keywords: Zebrafish; Temperature; Embryogenesis; Sex ratio; Sex determination; Colour pattern; Masculinization; Transcriptome analysis
 Abstract: BackgroundElevated water temperature, as is expected through climate change, leads to masculinization in fish species with sexual plasticity, resulting in changes in population dynamics. These changes are one important ecological consequence, contributing to the risk of extinction in small and inbred fish populations under natural conditions, due to male-biased sex ratio. Here we investigated the effect of elevated water temperature during embryogenesis on sex ratio and sex-biased gene expression profiles between two different tissues, namely gonad and caudal fin of adult zebrafish males and females, to gain new insights into the molecular mechanisms underlying sex determination (SD) and colour patterning related to sexual attractiveness.ResultsOur study demonstrated sex ratio imbalances with 25.5% more males under high-temperature condition, resulting from gonadal masculinization. The result of transcriptome analysis showed a significantly upregulated expression of male SD genes (e.g. dmrt1, amh, cyp11c1 and sept8b) and downregulation of female SD genes (e.g. zp2.1, vtg1, cyp19a1a and bmp15) in male gonads compared to female gonads. Contrary to expectations, we found highly differential expression of colour pattern (CP) genes in the gonads, suggesting the neofunctionalisation' of those genes in the zebrafish reproduction system. However, in the caudal fin, no differential expression of CP genes was identified, suggesting the observed differences in colouration between males and females in adult fish may be due to post-transcriptional regulation of key enzymes involved in pigment synthesis and distribution.ConclusionsOur study demonstrates male-biased sex ratio under high temperature condition and support a polygenic SD (PSD) system in laboratory zebrafish. We identify a subset of pathways (tight junction, gap junction and apoptosis), enriched for SD and CP genes, which appear to be co-regulated in the same pathway, providing evidence for involvement of those genes in the regulation of phenotypic sexual dimorphism in zebrafish.

Details

show
hide
Language(s): eng - English
 Dates: 2019-05-06
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1186/s12864-019-5722-1
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: BMC Genomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 17 Volume / Issue: 20 Sequence Number: 341 Start / End Page: - Identifier: -