English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Rapid mixing of colliding picoliter liquid droplets delivered through-space from piezoelectric-actuated pipettes characterized by time-resolved fluorescence monitoring

Wu, J. L. Y., Tellkamp, F., Khajehpour, M., Robertson, W., & Miller, R. J. D. (2019). Rapid mixing of colliding picoliter liquid droplets delivered through-space from piezoelectric-actuated pipettes characterized by time-resolved fluorescence monitoring. Review of Scientific Instruments, 90(5): 055109. doi:10.1063/1.5050270.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-B010-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-920F-7
Genre: Journal Article

Files

show Files
hide Files
:
1.5050270.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
1.5050270.pdf
Description:
-
Visibility:
Private (embargoed till 2020-05-21)
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
2019
Copyright Info:
© the Author(s)

Locators

show
hide
Locator:
https://dx.doi.org/10.1063/1.5050270 (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Wu, J. L. Y.1, 2, Author
Tellkamp, F.3, Author              
Khajehpour, M.4, Author
Robertson, W.2, 5, Author              
Miller, R. J. D.2, 6, Author              
Affiliations:
1Division of Engineering Science, University of Toronto, ou_persistent22              
2Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
3Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
4Department of Chemistry, University of Manitoba, ou_persistent22              
5Quantum Systems Division, Georgia Tech Research Institute, ou_persistent22              
6Departments of Chemistry and Physics, University of Toronto, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Rapid mixing of aqueous solutions is a crucial first step to study the kinetics of fast biochemical reactions with high temporal resolution. Remarkable progress toward this goal has been made through the development of advanced stopped-flow mixing techniques resulting in reduced dead times, and thereby extending reaction monitoring capabilities to numerous biochemical systems. Concurrently, piezoelectric actuators for through-space liquid droplet sample delivery have also been applied in several experimental systems, providing discrete picoliter sample volume delivery and precision sample deposition onto a surface, free of confinement within microfluidic devices, tubing, or other physical constraints. Here, we characterize the inertial mixing kinetics of two aqueous droplets (130 pl) produced by piezoelectric-actuated pipettes, following droplet collision in free space and deposition on a surface in a proof of principle experiment. A time-resolved fluorescence system was developed to monitor the mixing and fluorescence quenching of 5-carboxytetramethylrhodamine (5-Tamra) and N-Bromosuccinimide, which we show to occur in less than 10 ms. In this respect, this methodology is unique in that it offers millisecond mixing capabilities for very small quantities of discrete sample volumes. Furthermore, the use of discrete droplets for sample delivery and mixing in free space provides potential advantages, including the elimination of the requirement for a physical construction as with microfluidic systems, and thereby makes possible and extends the experimental capabilities of many systems.

Details

show
hide
Language(s): eng - English
 Dates: 2018-07-292019-04-272019-05-212019-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1063/1.5050270
 Degree: -

Event

show

Legal Case

show

Project information

show hide
Project name : This work was supported by the Max Planck Society. We would like to thank Jessica Besaw and William Stuart for their help in the assembly and testing of the Microdrop autopipette system.
Grant ID : -
Funding program : -
Funding organization : -

Source 1

show
hide
Title: Review of Scientific Instruments
  Abbreviation : Rev. Sci. Instrum.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Melville, NY : AIP Publishing
Pages: - Volume / Issue: 90 (5) Sequence Number: 055109 Start / End Page: - Identifier: ISSN: 0034-6748
CoNE: https://pure.mpg.de/cone/journals/resource/991042742033452