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  Prospects for visual proteomics using in situ cryo-electron tomography

Kämmerer, S. (2018). Prospects for visual proteomics using in situ cryo-electron tomography. PhD Thesis, Technische Universität, München.

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This thesis contributes to a daring vision of in situ cryo-electron tomography: Visual Proteomics, a comprehensive molecular atlas of the cell. Three detailed analyses of the green alga Chlamydomonas test the potential of (1) reaching functional conclusions by combining structural and positional information, (2) understanding a process through a structural interactome and (3) making new discoveries by structural analysis of hitherto unknown complexes.

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Kämmerer, Sahradha1, Author              
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1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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 Abstract: With the advent of focused ion beam milling and direct detectors, in situ cryo-electron tomography set off on a journey to explore structures deep within the cell. This thesis contributes to a daring vision of the future that in situ research can take: Visual Proteomics, a comprehensive molecular atlas of the cell. With the technical foundation laid, incremental steps towards Visual Proteomics were taken in three detailed analyses of the green alga Chlamydomonas, testing the potential of (1) reaching functional conclusions by combining structural and positional information, (2) understanding a process through a structural interactome and (3) making new discoveries by structural analyses of hitherto unknown complexes. The respective results included the (1) identification of a nuclear degradation hub around nuclear pore complexes composed of structurally tethered proteasomes, (2) localization of ER-associated degradation in non-membrane bound microcompartments of densely clustered proteasomes at the ER membrane and (3) structural description of trans-luminal protein arrays that link the central cisternae of the Golgi together. Concluding, there is a discussion of current advancements and required future developments towards the realization of Visual Proteomics.

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 Dates: 2018-08-29
 Publication Status: Accepted / In Press
 Pages: 113
 Publishing info: München : Technische Universität
 Table of Contents: -
 Rev. Type: -
 Identifiers: URN: nbn:de:bvb:91-diss-20180918-1443520-1-9
 Degree: PhD

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