English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Quantitative sensing and signalling of single-stranded DNA during the DNA damage response

Bantele, S. C. S., Lisby, M., & Pfander, B. (2019). Quantitative sensing and signalling of single-stranded DNA during the DNA damage response. Nature Communications, 10: 944. doi:10.1038/s41467-019-08889-5.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-E1F8-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-E1F9-6
Genre: Journal Article

Files

show Files
hide Files
:
s41467-019-08889-5.pdf (Publisher version), 2MB
Name:
s41467-019-08889-5.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
Open Access
:
41467_2019_8889_MOESM1_ESM.pdf (Supplementary material), 11MB
Name:
41467_2019_8889_MOESM1_ESM.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Bantele, Susanne C. S.1, Author              
Lisby, Michael2, Author
Pfander, Boris1, Author              
Affiliations:
1Pfander, Boris / DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565165              
2external, ou_persistent22              

Content

show
hide
Free keywords: REPLICATION PROTEIN-A; END RESECTION; HOMOLOGOUS RECOMBINATION; CHECKPOINT ACTIVATION; CHROMATIN DYNAMICS; MEC1 KINASE; HISTONE H2A; ATR-KINASE; BREAKS; REPAIRScience & Technology - Other Topics;
 Abstract: The DNA damage checkpoint senses the presence of DNA lesions and controls the cellular response thereto. A crucial DNA damage signal is single-stranded DNA (ssDNA), which is frequently found at sites of DNA damage and recruits the sensor checkpoint kinase Mec1-Ddc2. However, how this signal - and therefore the cell's DNA damage load - is quantified, is poorly understood. Here, we use genetic manipulation of DNA end resection to induce quantitatively different ssDNA signals at a site-specific double strand break in budding yeast and identify two distinct signalling circuits within the checkpoint. The local checkpoint signalling circuit leading to gamma H2A phosphorylation is unresponsive to increased amounts of ssDNA, while the global checkpoint signalling circuit, which triggers Rad53 activation, integrates the ssDNA signal quantitatively. The global checkpoint signal critically depends on the 9-1-1 and its downstream acting signalling axis, suggesting that ssDNA quantification depends on at least two sensor complexes.

Details

show
hide
Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 10 Sequence Number: 944 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723