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  Halobacterium salinarum virus ChaoS9, a Novel Halovirus Related to PhiH1 and PhiCh1

Dyall-Smith, M., Palm, P., Wanner, G., Witte, A., Oesterhelt, D., & Pfeiffer, F. (2019). Halobacterium salinarum virus ChaoS9, a Novel Halovirus Related to PhiH1 and PhiCh1. Genes, 10(3): 194. doi:10.3390/genes10030194.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-E9CB-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-E9CC-1
Genre: Journal Article

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 Creators:
Dyall-Smith, Mike1, Author              
Palm, Peter2, Author              
Wanner, Gerhard3, Author
Witte, Angela3, Author
Oesterhelt, Dieter2, Author              
Pfeiffer, Friedhelm1, Author              
Affiliations:
1Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1832284              
2Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565164              
3external, ou_persistent22              

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Free keywords: NUCLEOTIDE-SEQUENCE; ENORMOUS DIVERSITY; PHAGE; HALOBIUM; BACTERIORHODOPSIN; GENOME; BACTERIOPHAGES; MEMBRANE; PHI-CH1; GENEGenetics & Heredity; halovirus; caudovirus; halobacteria; Archaea; haloarchaea; genome inversion; transposon;
 Abstract: The unexpected lysis of a large culture of Halobacterium salinarum strain S9 was found to be caused by a novel myovirus, designated ChaoS9. Virus purification from the culture lysate revealed a homogeneous population of caudovirus-like particles. The viral genome is linear, dsDNA that is partially redundant and circularly permuted, has a unit length of 55,145 nt, a G + C% of 65.3, and has 85 predicted coding sequences (CDS) and one tRNA (Arg) gene. The left arm of the genome (0-28 kbp) encodes proteins similar in sequence to those from known caudoviruses and was most similar to myohaloviruses phiCh1 (host: Natrialba magadii) and phiH1 (host: Hbt. salinarum). It carries a tail-fiber gene module similar to the invertible modules present in phiH1 and phiCh1. However, while the tail genes of ChaoS9 were similar to those of phiCh1 and phiH1, the Mcp of ChaoS9 was most similar (36% aa identity) to that of Haloarcula hispanica tailed virus 1 (HHTV-1). Provirus elements related to ChaoS9 showed most similarity to tail/assembly proteins but varied in their similarity with head/assembly proteins. The right arm (29-55 kbp) of ChaoS9 encoded proteins involved in DNA replication (ParA, RepH, and Orc1) but the other proteins showed little similarity to those from phiH1, phiCh1, or provirus elements, and most of them could not be assigned a function. ChaoS9 is probably best classified within the genus Myohalovirus, as it shares many characteristics with phiH1 (and phiCh1), including many similar proteins. However, the head/assembly gene region appears to have undergone a recombination event, and the inferred proteins are different to those of phiH1 and phiCh1, including the major capsid protein. This makes the taxonomic classification of ChaoS9 more ambiguous. We also report a revised genome sequence and annotation of Natrialba virus phiCh1.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 23
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000464471300004
DOI: 10.3390/genes10030194
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Title: Genes
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 10 (3) Sequence Number: 194 Start / End Page: - Identifier: Other: 2073-4425
Other: http://sherpa.mimas.ac.uk/romeo/issn/2073-4425/
CoNE: https://pure.mpg.de/cone/journals/resource/2073-4425