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  Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination

Kamieniarz-Gdula, K., Gdula, M. R., Panser, K., Nojima, T., Monks, J., Wiśniewski, J. R., et al. (2019). Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination. Molecular Cell, 74(1), 158-172.e9. doi:10.1016/j.molcel.2019.01.027.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-E9E6-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-E9E7-2
Genre: Journal Article

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 Creators:
Kamieniarz-Gdula, Kinga1, Author
Gdula, Michal R.1, Author
Panser, Karin1, Author
Nojima, Takayuki1, Author
Monks, Joan1, Author
Wiśniewski, Jacek R.2, Author              
Riepsaame, Joey1, Author
Brockdorff, Neil1, Author
Pauli, Andrea1, Author
Proudfoot, Nick J.1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: RNA-POLYMERASE-II; PRE-MESSENGER-RNA; ALTERNATIVE POLYADENYLATION; ELONGATION COMPLEX; PROCESSING FACTOR; NONCODING RNA; NASCENT RNA; POL-II; CLEAVAGE; CTDBiochemistry & Molecular Biology; Cell Biology;
 Abstract: The pervasive nature of RNA polymerase II (Pol II) transcription requires efficient termination. A key player in this process is the cleavage and polyadenylation (CPA) factor PCF11, which directly binds to the Pol II C-terminal domain and dismantles elongating Pol II from DNA in vitro. We demonstrate that PCF11-mediated termination is essential for vertebrate development. A range of genomic analyses, including mNET-seq, 3' mRNA-seq, chromatin RNAseq, and ChIP-seq, reveals that PCF11 enhances transcription termination and stimulates early polyadenylation genome-wide. PCF11 binds preferentially between closely spaced genes, where it prevents transcriptional interference and consequent gene downregulation. Notably, PCF11 is sub-stoichiometric to the CPA complex. Low levels of PCF11 are maintained by an auto-regulatory mechanism involving premature termination of its own transcript and are important for normal development. Both in human cell culture and during zebrafish development, PCF11 selectively attenuates the expression of other transcriptional regulators by premature CPA and termination.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 24
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
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Title: Molecular Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 74 (1) Sequence Number: - Start / End Page: 158 - 172.e9 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929