English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Spatial proteomics: a powerful discovery tool for cell biology

Lundberg, E., & Borner, G. H. H. (2019). Spatial proteomics: a powerful discovery tool for cell biology. Nature Reviews Molecular Cell Biology, 20(5), 285-302. doi:10.1038/s41580-018-0094-y.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Lundberg, Emma1, Author
Borner, Georg H. H.2, Author              
Affiliations:
1external, ou_persistent22              
2Borner, Georg / Systems Biology of Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_3060205              

Content

show
hide
Free keywords: PROTEIN SUBCELLULAR-LOCALIZATION; MOONLIGHTING PROTEINS; MICROSCOPY IMAGES; HUMAN INTERACTOME; HUMAN CENTROSOME; DNA-DAMAGE; STEM-CELLS; ANTIBODY; YEAST; LIVECell Biology;
 Abstract: Protein subcellular localization is tightly controlled and intimately linked to protein function in health and disease. Capturing the spatial proteome - that is, the localizations of proteins and their dynamics at the subcellular level - is therefore essential for a complete understanding of cell biology. Owing to substantial advances in microscopy, mass spectrometry and machine learning applications for data analysis, the field is now mature for proteome-wide investigations of spatial cellular regulation. Studies of the human proteome have begun to reveal a complex architecture, including single-cell variations, dynamic protein translocations, changing interaction networks and proteins localizing to multiple compartments. Furthermore, several studies have successfully harnessed the power of comparative spatial proteomics as a discovery tool to unravel disease mechanisms. We are at the beginning of an era in which spatial proteomics finally integrates with cell biology and medical research, thereby paving the way for unbiased systems-level insights into cellular processes. Here, we discuss current methods for spatial proteomics using imaging or mass spectrometry and specifically highlight global comparative applications. The aim of this Review is to survey the state of the field and also to encourage more cell biologists to apply spatial proteomics approaches.

Details

show
hide
Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 18
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000465500200008
DOI: 10.1038/s41580-018-0094-y
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Reviews Molecular Cell Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London, UK : Nature Pub. Group
Pages: - Volume / Issue: 20 (5) Sequence Number: - Start / End Page: 285 - 302 Identifier: ISSN: 1471-0072
CoNE: https://pure.mpg.de/cone/journals/resource/110985821000941