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  The proteasome biogenesis regulator Rpn4 cooperates with the unfolded protein response to promote ER stress resistance

Schmidt, R. M., Schessner, J. P., Borner, G., & Schuck, S. (2019). The proteasome biogenesis regulator Rpn4 cooperates with the unfolded protein response to promote ER stress resistance. eLife, 8: e43244. doi:10.7554/eLife.43244.

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 Creators:
Schmidt, Rolf M.1, Author
Schessner, Julia P.2, Author              
Borner, Georg2, Author              
Schuck, Sebastian1, Author
Affiliations:
1external, ou_persistent22              
2Borner, Georg / Systems Biology of Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_3060205              

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Free keywords: ENDOPLASMIC-RETICULUM STRESS; HEAT-SHOCK RESPONSE; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTION FACTOR; QUALITY-CONTROL; MEMBRANE-PROTEINS; 26S PROTEASOME; YEAST; DEGRADATION; GENESLife Sciences & Biomedicine - Other Topics;
 Abstract: Misfolded proteins in the endoplasmic reticulum (ER) activate the unfolded protein response (U PR), which enhances protein folding to restore homeostasis. Additional pathways respond to ER stress, but how they help counteract protein misfolding is incompletely understood. Here, we develop a titratable system for the induction of ER stress in yeast to enable a genetic screen for factors that augment stress resistance independently of the UPR. We identify the proteasome biogenesis regulator Rpn4 and show that it cooperates with the UPR. Rpn4 abundance increases during ER stress, first by a post-transcriptional, then by a transcriptional mechanism. Induction of RPN4 transcription is triggered by cytosolic mislocalization of secretory proteins, is mediated by multiple signaling pathways and accelerates clearance of misfolded proteins from the cytosol. Thus, Rpn4 and the UPR are complementary elements of a modular cross-compartment response to ER stress.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 28
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000461162600001
DOI: 10.7554/eLife.43244
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Project name : MA 1764/2-1
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Funding organization : Deutsche Forschungsgemeinschaft

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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 8 Sequence Number: e43244 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X