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  124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics

Wade, O. K., Woehrstein, J. B., Nickels, P. C., Strauss, S., Stehr, F., Stein, J., et al. (2019). 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics. Nano Letters, 19(4), 2641-2646. doi:10.1021/acs.nanolett.9b00508.

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 Creators:
Wade, Orsolya K.1, Author              
Woehrstein, Johannes B.1, Author              
Nickels, Philipp C.1, Author              
Strauss, Sebastian1, Author              
Stehr, Florian2, Author              
Stein, Johannes2, Author              
Schueder, Florian1, Author              
Strauss, Maximilian T.1, Author              
Ganji, Mahipal1, Author              
Schnitzbauer, Joerg1, Author              
Grabmayr, Heinrich1, Author              
Yin, Peng3, Author
Schwille, Petra2, Author              
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              
3external, ou_persistent22              

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Free keywords: MICROSCOPY; MOLECULESChemistry; Science & Technology - Other Topics; Materials Science; Physics; Super-resolution microscopy; DNA nanotechnology; DNA-PAINT; barcoding multiplexing;
 Abstract: Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: We thank Martin Spitaler and the imaging facility of the MPI of Biochemistry for confocal imaging support.
 Rev. Type: -
 Degree: -

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Project name : Marie Skłodowska-Curie grant agreement
Grant ID : 796606
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 19 (4) Sequence Number: - Start / End Page: 2641 - 2646 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403