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  MaxQuant. Live Enables Global Targeting of More Than 25,000 Peptides

Wichmann, C., Meier, F., Virreira Winter, S., Brunner, A.-D., Cox, J., & Mann, M. (2019). MaxQuant. Live Enables Global Targeting of More Than 25,000 Peptides. Molecular and Cellular Proteomics, 18(5), 982-994. doi:10.1074/mcp.TIR118.001131.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-DE7F-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-DE80-2
Genre: Journal Article

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 Creators:
Wichmann, Christoph1, Author              
Meier, Florian2, Author              
Virreira Winter, Sebastian2, Author              
Brunner, Andreas-David2, Author              
Cox, Jürgen1, Author              
Mann, Matthias2, Author              
Affiliations:
1Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_2063284              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: TANDEM MASS-SPECTROMETRY; CELL-CULTURE; AMINO-ACIDS; PROTEOMICS; DISCOVERY; RESOURCEBiochemistry & Molecular Biology;
 Abstract: Mass spectrometry (MS)-based proteomics is often performed in a shotgun format, in which as many peptide precursors as possible are selected from full or MS1 scans so that their fragment spectra can be recorded in MS2 scans. Although achieving great proteome depths, shotgun proteomics cannot guarantee that each precursor will be fragmented in each run. In contrast, targeted proteomics aims to reproducibly and sensitively record a restricted number of precursor/ fragment combinations in each run, based on prescheduled mass-to-charge and retention time windows. Here we set out to unify these two concepts by a global targeting approach in which an arbitrary number of precursors of interest are detected in real-time, followed by standard fragmentation or advanced peptide-specific analyses. We made use of a fast application programming interface to a quadrupole Orbitrap instrument and real-time recalibration in mass, retention time and intensity dimensions to predict precursor identity. MaxQuant. Live is freely available (www. maxquant. live) and has a graphical user interface to specify many predefined data acquisition strategies. Acquisition speed is as fast as with the vendor software and the power of our approach is demonstrated with the acquisition of breakdown curves for hundreds of precursors of interest. We also uncover precursors that are not even visible in MS1 scans, using elution time prediction based on the auto-adjusted retention time alone. Finally, we successfully recognized and targeted more than 25,000 peptides in single LC-MS runs. Global targeting combines the advantages of two classical approaches in MS-based proteomics, whereas greatly expanding the analytical toolbox. Molecular & Cellular Proteomics 18: 982-994, 2019. DOI: 10.1074/ mcp. TIR118.001131.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000467185700012
DOI: 10.1074/mcp.TIR118.001131
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Grant ID : 686547
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Funding organization : European Commission (EC)

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Title: Molecular and Cellular Proteomics
Source Genre: Journal
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Publ. Info: Bethesda, MD : American Society for Biochemistry and Molecular Biology
Pages: - Volume / Issue: 18 (5) Sequence Number: - Start / End Page: 982 - 994 Identifier: ISSN: 1535-9476
CoNE: https://pure.mpg.de/cone/journals/resource/111035577487002