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  MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording

Chen, Y., Pais-Roldán, P., Chen, X., Frosz, M., & Yu, X. (2019). MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording. Nature Communications, 10(1): 2536, pp. 1-11. doi:10.1038/s41467-019-10450-3.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-C3E2-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-C3E7-C
Genre: Journal Article

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 Creators:
Chen, Y1, 2, Author              
Pais-Roldán, P1, 2, Author              
Chen, X1, 2, Author              
Frosz, MH, Author
Yu, X1, 2, Author              
Affiliations:
1Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              
2Research Group Translational Neuroimaging and Neural Control, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_2528695              

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 Abstract: Optical fiber-mediated optogenetic activation and neuronal Ca2+ recording in combination with fMRI provide a multi-modal fMRI platform. Here, we developed an MRI-guided robotic arm (MgRA) as a flexible positioning system with high precision to real-time assist optical fiber brain intervention for multi-modal animal fMRI. Besides the ex vivo precision evaluation, we present the highly reliable brain activity patterns in the projected basal forebrain regions upon MgRA-driven optogenetic stimulation in the lateral hypothalamus. Also, we show the step-wise optical fiber targeting thalamic nuclei and map the region-specific functional connectivity with whole-brain fMRI accompanied by simultaneous calcium recordings to specify its circuit-specificity. The MgRA also guides the real-time microinjection to specific deep brain nuclei, which is demonstrated by an Mn-enhanced MRI method. The MgRA represents a clear advantage over the standard stereotaxic-based fiber implantation and opens a broad avenue to investigate the circuit-specific functional brain mapping with the multi-modal fMRI platform.

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 Dates: 2019-06
 Publication Status: Published online
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 Identifiers: DOI: 10.1038/s41467-019-10450-3
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 10 (1) Sequence Number: 2536 Start / End Page: 1 - 11 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723