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  The impact of mechanically stimulated muscle-derived stromal cells on aged skeletal muscle

Huntsman, H. D., Rendeiro, C., Merritt, J., Pincu, Y., Cobert, A., De Lisio, M., et al. (2018). The impact of mechanically stimulated muscle-derived stromal cells on aged skeletal muscle. Experimental Gerontology, 103, 35-46. doi:10.1016/j.exger.2017.12.012.

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 Creators:
Huntsman, Heather D., Author
Rendeiro, Catarina, Author
Merritt, Jennifer, Author
Pincu, Yair, Author
Cobert, Adam, Author
De Lisio, Michael, Author
Kolyvas, Emily, Author
Dvoretskiy, Svyatoslav, Author
Dobrucki, Iwona, Author
Kemkemer, Ralf1, Author           
Jensen, Tor, Author
Dobrucki, Lawrence, Author
Rhodes, Justin, Author
Boppart, Marni, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: Aging; Cognition; Exercise; Mesenchymal stem cells; Neurogenesis; Perivascular stromal cells; Skeletal muscle; Vascular perfusion
 Abstract: Perivascular stromal cells, including mesenchymal stem/stromal cells (MSCs), secrete paracrine factor in response to exercise training that can facilitate improvements in muscle remodeling. This study was designed to test the capacity for muscle-resident MSCs (mMSCs) isolated from young mice to release regenerative proteins in response to mechanical strain in vitro, and subsequently determine the extent to which strain-stimulated mMSCs can enhance skeletal muscle and cognitive performance in a mouse model of uncomplicated aging. Protein arrays confirmed a robust increase in protein release at 24h following an acute bout of mechanical strain in vitro (10%, 1Hz, 5h) compared to non-strain controls. Aged (24month old), C57BL/6 mice were provided bilateral intramuscular injection of saline, non-strain control mMSCs, or mMSCs subjected to a single bout of mechanical strain in vitro (4×104). No significant changes were observed in muscle weight, myofiber size, maximal force, or satellite cell quantity at 1 or 4wks between groups. Peripheral perfusion was significantly increased in muscle at 4wks post-mMSC injection (p<0.05), yet no difference was noted between control and preconditioned mMSCs. Intramuscular injection of preconditioned mMSCs increased the number of new neurons and astrocytes in the dentate gyrus of the hippocampus compared to both control groups (p<0.05), with a trend toward an increase in water maze performance noted (p=0.07). Results from this study demonstrate that acute injection of exogenously stimulated muscle-resident stromal cells do not robustly impact aged muscle structure and function, yet increase the survival of new neurons in the hippocampus.

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Language(s): eng - English
 Dates: 2017-11-162017-08-302017-12-142017-12-182018-03
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.exger.2017.12.012
 Degree: -

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Title: Experimental Gerontology
  Other : Exp. Gerontology
Source Genre: Journal
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Publ. Info: Oxford : Pergamon
Pages: - Volume / Issue: 103 Sequence Number: - Start / End Page: 35 - 46 Identifier: ISSN: 0531-5565
CoNE: https://pure.mpg.de/cone/journals/resource/954925530945