English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Optimization of insect odorant receptor trafficking and functional expression via transient transfection in HEK293 cells

Miazzi, F., Hoyer, C., Sachse, S., Knaden, M., Wicher, D., Hansson, B. S., et al. (2019). Optimization of insect odorant receptor trafficking and functional expression via transient transfection in HEK293 cells. Chemical Senses, 44(9), 673-682. doi:10.1093/chemse/bjz062.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
HAN335s1.pdf (Supplementary material), 2MB
Name:
HAN335s1.pdf
Description:
-
OA-Status:
Not specified
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
HAN335.pdf (Publisher version), 13MB
Name:
HAN335.pdf
Description:
-
OA-Status:
Not specified
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show
hide
Locator:
http://dx.doi.org/10.1093/chemse/bjz062 (Publisher version)
Description:
OA
OA-Status:
Not specified

Creators

show
hide
 Creators:
Miazzi, Fabio1, 2, Author           
Hoyer, Carolin1, Author           
Sachse, Silke3, Author           
Knaden, Markus4, Author           
Wicher, Dieter1, Author           
Hansson, Bill S.1, Author           
Lavista Llanos, Sofia5, Author           
Affiliations:
1Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421894              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
3Research Group Dr. S. Sachse, Olfactory Coding, MPI for Chemical Ecology, Max Planck Society, ou_421914              
4Research Group Dr. M. Knaden, Insect Behavior, Department of Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421913              
5Research Group Dr. M. Stensmyr, Molecular Biology of Olfaction, Department of Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421915              

Content

show
hide
Free keywords: -
 Abstract: Insect odorant receptors show a limited functional expression in various heterologous expression systems including insect and mammalian cells. This may be in part due to the absence of key components driving the release of these proteins from the endoplasmic reticulum and directing them to the plasma membrane. In order to mitigate this problem we took advantage of small export signals within the human HCN1 and Rhodopsin that have been shown to promote protein release from the endoplasmic reticulum and the trafficking of post-Golgi vesicles, respectively. Moreover, we designed a new vector based on a bidirectional expression cassette to drive the functional expression of the insect odorant receptor co-receptor (Orco) and an odor-binding odorant receptor, simultaneously. We show that this new method can be used to reliably express insect odorant receptors in HEK293 cells via transient transfection and that is highly suitable for downstream applications using automated and high-throughput imaging platforms.

Details

show
hide
Language(s):
 Dates: 2019-08-3120192019-10-26
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: HAN335
DOI: 10.1093/chemse/bjz062
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Chemical Senses
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 44 (9) Sequence Number: - Start / End Page: 673 - 682 Identifier: ISSN: 0379-864X
CoNE: https://pure.mpg.de/cone/journals/resource/954928560444