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  EF-G-induced ribosome sliding along the noncoding mRNA.

Klimova, M., Senyushkina, T., Samatova, E. N., Peng, B. Z., Pearson, M., Peske, F., et al. (2019). EF-G-induced ribosome sliding along the noncoding mRNA. Science Advances, 5(6): eaaw9049. doi:10.1126/sciadv.aaw9049.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-CC45-A Version Permalink: http://hdl.handle.net/21.11116/0000-0003-CC49-6
Genre: Journal Article

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 Creators:
Klimova, M.1, Author              
Senyushkina, T.1, Author              
Samatova, E. N.1, Author              
Peng, B. Z.1, Author              
Pearson, M.1, Author              
Peske, F.1, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

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 Abstract: Translational bypassing is a recoding event during which ribosomes slide over a noncoding region of the messenger RNA (mRNA) to synthesize one protein from two discontinuous reading frames. Structures in the mRNA orchestrate forward movement of the ribosome, but what causes ribosomes to start sliding remains unclear. Here, we show that elongation factor G (EF-G) triggers ribosome take-off by a pseudotranslocation event using a small mRNA stem-loop as an A-site transfer RNA mimic and requires hydrolysis of about two molecules of guanosine 5'-triphosphate per nucleotide of the noncoding gap. Bypassing ribosomes adopt a hyper-rotated conformation, also observed with ribosomes stalled by the SecM sequence, suggesting common ribosome dynamics during translation stalling. Our results demonstrate a new function of EF-G in promoting ribosome sliding along the mRNA, in contrast to codon-wise ribosome movement during canonical translation, and suggest a mechanism by which ribosomes could traverse untranslated parts of mRNAs.

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Language(s): eng - English
 Dates: 2019-06-05
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1126/sciadv.aaw9049
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Title: Science Advances
Source Genre: Journal
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Pages: 9 Volume / Issue: 5 (6) Sequence Number: eaaw9049 Start / End Page: - Identifier: -