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  Proteome profiling by label‐free mass spectrometry reveals differentiated response of Campylobacter jejuni 81–176 to sublethal concentrations of bile acids.

Masanta, W. O., Zautner, A. E., Lugert, R., Bohne, W., Gross, U., Leha, A., et al. (2019). Proteome profiling by label‐free mass spectrometry reveals differentiated response of Campylobacter jejuni 81–176 to sublethal concentrations of bile acids. Proteomics, 13(3): 1800083. doi:10.1002/prca.201800083.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-D112-C Version Permalink: http://hdl.handle.net/21.11116/0000-0003-D119-5
Genre: Journal Article

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 Creators:
Masanta, W. O., Author
Zautner, A. E., Author
Lugert, R., Author
Bohne, W., Author
Gross, U., Author
Leha, A., Author
Dakna, M., Author
Lenz, C.1, Author              
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: bile acids; Campylobacter jejuni; mass spectrometry; proteome; SWATH
 Abstract: Purpose Bile acids are crucial components of the intestinal antimicrobial defense and represent a significant stress factor for enteric pathogens. Adaptation processes of Campylobacter jejuni to this hostile environment are analyzed in this study by a proteomic approach. Experimental design Proteome profiling by label-free mass spectrometry (SWATH-MS) has been used to characterize the adaptation of C. jejuni to sublethal concentrations of seven bile acids. Results The bile acids with the lowest inhibitory concentration (IC50), deoxycholic and chenodeoxycholic acid, induce the most significant proteome changes. Overall a downregulation of all basic biosynthetic pathways and a general decrease in the transcription machinery are found. Concurrently, an induction of factors involved in detoxification of reactive oxygen species, protein folding, and bile acid exporting efflux pumps is detected. Exposure to deoxycholic and chenodeoxycholic acid results in an increased expression of components of the more energy-efficient aerobic respiration pathway, while the anaerobic branches of the electron transport chain are down-expressed. Conclusions and clinical relevance The results show that C. jejuni has a differentiated system of adaptation to bile acid stresses. The findings enhance the understanding of the pathogenesis of campylobacteriosis, especially for survival of C. jejuni in the human intestine, and may provide clues to future medical treatment.

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Language(s): eng - English
 Dates: 2018-09-242019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/prca.201800083
 Degree: -

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Title: Proteomics
Source Genre: Journal
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Pages: 12 Volume / Issue: 13 (3) Sequence Number: 1800083 Start / End Page: - Identifier: -