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  Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain

Lee, D., Creed, M., Jung, K., Stefanelli, T., Wendler, D. J., Oh, W. C., et al. (2017). Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Nature Methods, 495-503. Retrieved from http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.4234.html.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0003-D55B-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0005-B8FC-0
Genre: Journal Article
Alternative Title : Nature Methods

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 Creators:
Lee, Dongmin1, Author
Creed, Meaghan, Author
Jung, Kanghoon1, Author
Stefanelli, Thomas, Author
Wendler, Daniel J.1, Author
Oh, Won Chan1, Author
Mignocchi, Neymi Layne1, Author
Lüscher, Christian, Author
Kwon, Hyung-Bae1, Author
Affiliations:
1Max Planck Florida Institute for Neuroscience, Max Planck Society, One Max Planck Way, Jupiter FL 33458, USA, ou_1950288              

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Free keywords: Neuroscience, Optogenetics, Model vertebrates
 Abstract: Few tools exist to visualize and manipulate neurons that are targets of neuromodulators. We present iTango, a light- and ligand-gated gene expression system based on a light-inducible split tobacco etch virus protease. Cells expressing the iTango system exhibit increased expression of a marker gene in the presence of dopamine and blue-light exposure, both in vitro and in vivo. We demonstrated the iTango system in a behaviorally relevant context, by inducing expression of optogenetic tools in neurons under dopaminergic control during a behavior of interest. We thereby gained optogenetic control of these behaviorally relevant neurons. We applied the iTango system to decipher the roles of two classes of dopaminergic neurons in the mouse nucleus accumbens in a sensitized locomotor response to cocaine. Thus, the iTango platform allows for control of neuromodulatory circuits in a genetically and functionally defined manner with spatial and temporal precision.

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 Dates: 2017
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: URI: http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.4234.html
URI: http://www.nature.com/nmeth/journal/vaop/ncurrent/pdf/nmeth.4234.pdf
URI: http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.4234.html
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Title: Nature Methods
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 495 - 503 Identifier: -