Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  Video-rate volumetric functional imaging of the brain at synaptic resolution

Lu, R., Sun, W., Liang, Y., Kerlin, A., Bierfeld, J., Seelig, J. D., et al. (2017). Video-rate volumetric functional imaging of the brain at synaptic resolution. Nature Neuroscience. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/28250408.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0003-D51E-C Versions-Permalink: https://hdl.handle.net/21.11116/0000-0003-D51F-B
Genre: Zeitschriftenartikel
Alternativer Titel : Nature Neuroscience

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Lu, Rongwen, Autor
Sun, Wenzhi, Autor
Liang, Yajie, Autor
Kerlin, Aaron, Autor
Bierfeld, Jens, Autor
Seelig, Johannes D., Autor
Wilson, Daniel E.1, Autor
Scholl, Benjamin1, Autor
Mohar, Boaz, Autor
Tanimoto, Masashi, Autor
Koyama, Minoru, Autor
Fitzpatrick, David1, Autor
Orger, Michael B., Autor
Ji, Na, Autor
Affiliations:
1Kunsthistorisches Institut in Florenz, MPI, Max Planck Society, Via Giuseppe Giusti 44, 50121 Florence, Italy, ou_1254545              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Neurons and neural networks often extend hundreds of micrometers in three dimensions. Capturing the calcium transients associated with their activity requires volume imaging methods with subsecond temporal resolution. Such speed is a challenge for conventional two-photon laser-scanning microscopy, because it depends on serial focal scanning in 3D and indicators with limited brightness. Here we present an optical module that is easily integrated into standard two-photon laser-scanning microscopes to generate an axially elongated Bessel focus, which when scanned in 2D turns frame rate into volume rate. We demonstrated the power of this approach in enabling discoveries for neurobiology by imaging the calcium dynamics of volumes of neurons and synapses in fruit flies, zebrafish larvae, mice and ferrets in vivo. Calcium signals in objects as small as dendritic spines could be resolved at video rates, provided that the samples were sparsely labeled to limit overlap in their axially projected images.

Details

einblenden:
ausblenden:
Sprache(n):
 Datum: 2017
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: URI: http://www.ncbi.nlm.nih.gov/pubmed/28250408
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Nature Neuroscience
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: - Identifikator: -