English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Sclerotinia sclerotiorum circumvents flavonoid defenses by catabolizing flavonol glycosides and aglycones

Chen, J., Ullah, C., Reichelt, M., Gershenzon, J., & Hammerbacher, A. (2019). Sclerotinia sclerotiorum circumvents flavonoid defenses by catabolizing flavonol glycosides and aglycones. Plant Physiology, 180(4), 1975-1987. doi:10.1104/pp.19.00461.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
GER566.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
GER566.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
GER566s1.pdf (Supplementary material), 913KB
 
File Permalink:
-
Name:
GER566s1.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://doi.org/10.1104/pp.19.00461 (Publisher version)
Description:
OA

Creators

show
hide
 Creators:
Chen, Jingyuan1, 2, Author              
Ullah, Chhana1, 2, Author              
Reichelt, Michael1, Author              
Gershenzon, Jonathan1, Author              
Hammerbacher, Almuth, Author
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

Content

show
hide
Free keywords: -
 Abstract: Flavonols are widely distributed plant metabolites that inhibit microbial growth. Yet many pathogens cause disease in flavonolcontaining plant tissues. We investigated how Sclerotinia sclerotiorum, a necrotrophic fungal pathogen that causes disease in a range of economically important crop species, is able to successfully infect flavonol-rich tissues of Arabidopsis (Arabidopsis thaliana). Infection of rosette stage Arabidopsis with a virulent S. sclerotiorum strain led to the selective hydrolysis of flavonol glycosidic linkages and the inducible degradation of flavonol aglycones to phloroglucinol carboxylic and phenolic acids. By chemical analysis of fungal biotransformation products and a search of the S. sclerotiorum genome sequence, we identified a quercetin dioxygenase gene (QDO) and characterized the encoded protein, which catalyzed cleavage of the flavonol carbon skeleton. QDO deletion lines degraded flavonols with much lower efficiency and were less pathogenic on Arabidopsis leaves than wild-type S. sclerotiorum, indicating the importance of flavonol degradation in fungal virulence. In the absence of QDO, flavonols exhibited toxicity toward S. sclerotiorum, demonstrating the potential roles of these phenolic compounds in protecting plants against pathogens.

Details

show
hide
Language(s):
 Dates: 2019-05-262019-082019-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: GER566
DOI: 10.1104/pp.19.00461
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 180 (4) Sequence Number: - Start / End Page: 1975 - 1987 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438