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  Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP)

Koch, H. R., Blohm-Sievers, E., & Liedvogel, M. (2019). Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP). Ecology and Evolution, 9(10), 5849-5858. doi:10.1002/ece3.5168.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-472B-D Version Permalink: http://hdl.handle.net/21.11116/0000-0004-472C-C
Genre: Journal Article

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Koch_et_al-2019-Ecology_and_Evolution.pdf (Publisher version), 3MB
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 Creators:
Koch, Hanna R.1, Author              
Blohm-Sievers, Elke, Author              
Liedvogel, Miriam1, Author              
Affiliations:
1Max Planck Research Group Behavioural Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2129640              

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Free keywords: blackcap, CHD-W, CHD-Z, Loop-mediated isothermal amplification, molecular sexing, passerine, sex determination, Sylvia atricapilla
 Abstract: Abstract Many bird species are sexually monomorphic and cannot be sexed based on phenotypic traits. Rapid sex determination is often a necessary component of avian studies focusing on behavior, ecology, evolution, and conservation. While PCR-based methods are the most common technique for molecularly sexing birds in the laboratory, a simpler, faster, and cheaper method has emerged, which can be used in the laboratory, but importantly also in the field. Herein, we used loop-mediated isothermal amplification (LAMP) for rapid sex determination of blood samples from juvenile European blackcaps, Sylvia atricapilla, sampled in the wild. We designed LAMP primers unique to S. atricapilla based on the sex chromosome-specific gene, chromo-helicase-DNA-binding protein (CHD), optimized the primers for laboratory and field application, and then used them to test a subset of wild-caught juvenile blackcaps of unknown gender at the time of capture. Sex determination results were fast and accurate. The advantages of this technique are that it allows researchers to identify the sex of individual birds within hours of sampling and eliminates the need for direct access to a laboratory if implemented at a remote field site. This work adds to the increasing list of available LAMP primers for different bird species and is a new addition within the Passeriformes order.

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Language(s): eng - English
 Dates: 2019-03-142018-10-172019-03-222019-04-152019-05
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/ece3.5168
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Title: Ecology and Evolution
Source Genre: Journal
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Publ. Info: John Wiley & Sons, Inc.
Pages: - Volume / Issue: 9 (10) Sequence Number: - Start / End Page: 5849 - 5858 Identifier: ISSN: 2045-7758
CoNE: https://pure.mpg.de/cone/journals/resource/2045-7758