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  Protein-level assembly increases protein sequence recovery from metagenomic samples manyfold.

Steinegger, M., Mirdita, M., & Söding, J. (2019). Protein-level assembly increases protein sequence recovery from metagenomic samples manyfold. Nature Methods, 16, 603-606. doi:10.1038/s41592-019-0437-4.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0003-E0DD-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0003-E0E1-1
Genre: Journal Article

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 Creators:
Steinegger, M.1, Author           
Mirdita, M.1, Author           
Söding, J.1, Author           
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1Research Group of Computational Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1933286              

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 Abstract: The open-source de novo protein-level assembler, Plass ( https://plass.mmseqs.com ), assembles six-frame-translated sequencing reads into protein sequences. It recovers 2-10 times more protein sequences from complex metagenomes and can assemble huge datasets. We assembled two redundancy-filtered reference protein catalogs, 2 billion sequences from 640 soil samples (soil reference protein catalog) and 292 million sequences from 775 marine eukaryotic metatranscriptomes (marine eukaryotic reference catalog), the largest free collections of protein sequences.

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Language(s): eng - English
 Dates: 2019-06-242019-07
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-019-0437-4
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Title: Nature Methods
Source Genre: Journal
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Pages: - Volume / Issue: 16 Sequence Number: - Start / End Page: 603 - 606 Identifier: -