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  Structural basis of TFIIH activation for nucleotide excision repair.

Kokic, G., Chernev, A., Tegunov, D., Dienemann, C., Urlaub, H., & Cramer, P. (2019). Structural basis of TFIIH activation for nucleotide excision repair. Nature Communications, 10: 2885. doi:10.1038/s41467-019-10745-5.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-E686-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-E68D-B
Genre: Journal Article

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 Creators:
Kokic, G.1, Author              
Chernev, A.2, Author              
Tegunov, D.1, Author              
Dienemann, C.1, Author              
Urlaub, H.2, Author              
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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 Abstract: Nucleotide excision repair (NER) is the major DNA repair pathway that removes UV-induced and bulky DNA lesions. There is currently no structure of NER intermediates, which form around the large multisubunit transcription factor IIH (TFIIH). Here we report the cryo-EM structure of an NER intermediate containing TFIIH and the NER factor XPA. Compared to its transcription conformation, the TFIIH structure is rearranged such that its ATPase subunits XPB and XPD bind double- and single-stranded DNA, consistent with their translocase and helicase activities, respectively. XPA releases the inhibitory kinase module of TFIIH, displaces a 'plug' element from the DNA-binding pore in XPD, and together with the NER factor XPG stimulates XPD activity. Our results explain how TFIIH is switched from a transcription to a repair factor, and provide the basis for a mechanistic analysis of the NER pathway.

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Language(s): eng - English
 Dates: 2019-06-28
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-019-10745-5
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Title: Nature Communications
Source Genre: Journal
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Pages: 9 Volume / Issue: 10 Sequence Number: 2885 Start / End Page: - Identifier: -