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  A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

So, C., Seres, B., Steyer, A. M., Mönnich, E., Clift, D., Pejkovska, A., et al. (2019). A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science, 364(6447): eaat9557. doi:10.1126/science.aat9557.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-E69C-A Version Permalink: http://hdl.handle.net/21.11116/0000-0003-E69F-7
Genre: Journal Article

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So, C.1, Author              
Seres, B.1, Author              
Steyer, A. M., Author
Mönnich, E., Author
Clift, D., Author
Pejkovska, A., Author
Möbius, W., Author
Schuh, M.1, Author              
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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 Abstract: Mammalian oocytes segregate chromosomes with a microtubule spindle that lacks centrosomes, but the mechanisms by which acentrosomal spindles are organized and function are largely unclear. In this study, we identify a conserved subcellular structure in mammalian oocytes that forms by phase separation. This structure, which we term the liquid-like meiotic spindle domain (LISD), permeates the spindle poles and forms dynamic protrusions that extend well beyond the spindle. The LISD selectively concentrates multiple microtubule regulatory factors and allows them to diffuse rapidly within the spindle volume. Disruption of the LISD via different means disperses these factors and leads to severe spindle assembly defects. Our data suggest a model whereby the LISD promotes meiotic spindle assembly by serving as a reservoir that sequesters and mobilizes microtubule regulatory factors in proximity to spindle microtubules.

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Language(s): eng - English
 Dates: 2019-06-28
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.aat9557
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Title: Science
Source Genre: Journal
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Pages: 14 Volume / Issue: 364 (6447) Sequence Number: eaat9557 Start / End Page: - Identifier: -