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  Snap-heated freeze-free preservation and processing of the urine proteome using the combination of stabilizor-based technology and filter aided sample preparation

Carvalho, L. B., Capelo-Martinez, J. L., Lodeiro, C., Wisniewski, J. R., & Santos, H. M. (2019). Snap-heated freeze-free preservation and processing of the urine proteome using the combination of stabilizor-based technology and filter aided sample preparation. Analytica Chimica Acta, 1076, 82-90. doi:10.1016/j.aca.2019.05.051.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-E774-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-E775-5
Genre: Journal Article

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 Creators:
Carvalho, Luis B.1, Author
Capelo-Martinez, Jose Luis1, Author
Lodeiro, Carlos1, Author
Wisniewski, Jacek R.2, Author              
Santos, Hugo M.1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: BODY-FLUID PROTEOMICS; BIOMARKER DISCOVERY; CLINICAL-SAMPLES; KIDNEY INJURY; PROTEINS; STORAGE; TEMPERATURE; MEMBRANE; PLATFORM; PERSEUSChemistry; Urine proteomics; Room temperature urine preservation; Heat stabilization; Filter-aided sample preparation; Label-free quantitative mass spectrometry;
 Abstract: We present a method to preserve and process urine proteins for proteomic analysis in a filter aided sample preparation (FASP) format. The method combines concentration of urine proteins in ultrafiltration devices, their thermal stabilization, allowing long term storage of the samples, and filter aided sample preparation. Proteomes of four different urines were preserved during 48 h and 6 months using (i) the classic freeze preservation at -20 degrees C, (ii) snap-heated freeze-free preservation at laboratory temperature (20 degrees C) and (iii) snap-heated preservation at -60 degrees C. The three storage methods can effetely preserve the urine proteome for at least 6 months without significant alterations. Abundances of more than 500 proteins and specially 24 selected -cleared or -approved protein assayed in serum or plasma were found similar within the three preservation methods assessed. The new method here proposed dramatically simplifies the conditions for preserving the urine proteome for biobanks in terms of space and storage, including lowering the risks of sample degradation caused by misfunction of the freezer. Furthermore, the shipping of large number of samples can be made without the need of freezing. The application of the FASP format to isolate and preserve the proteins facilitates long-term storage and processing of proteome of urine samples. (C) 2019 Elsevier B.V. All rights reserved.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000471599900008
DOI: 10.1016/j.aca.2019.05.051
 Degree: -

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Title: Analytica Chimica Acta
  Abbreviation : Anal. Chim. Acta
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1076 Sequence Number: - Start / End Page: 82 - 90 Identifier: ISSN: 0003-2670
CoNE: https://pure.mpg.de/cone/journals/resource/954925379952