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  Biogenic regions of cyanobacterial thylakoids form contact sites with the plasma membrane

Rast, A., Schaffer, M., Albert, S., Wan, W., Pfeffer, S., Beck, F., et al. (2019). Biogenic regions of cyanobacterial thylakoids form contact sites with the plasma membrane. Nature Plants, 5(4), 436-446. doi:10.1038/s41477-019-0399-7.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-E975-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-E976-2
Genre: Journal Article

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 Creators:
Rast, Anna1, Author
Schaffer, Miroslava2, Author              
Albert, Sahradha2, Author              
Wan, William2, Author              
Pfeffer, Stefan1, Author
Beck, Florian2, Author              
Plitzko, Jürgen M.2, Author              
Nickelsen, Joerg1, Author
Engel, Benjamin D.2, Author              
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: PHOTOSYSTEM-II; CRYOELECTRON TOMOGRAPHY; 3-DIMENSIONAL STRUCTURE; ENERGY-TRANSFER; INITIAL STEPS; PHYCOBILISOME; ULTRASTRUCTURE; VISUALIZATION; ORGANIZATION; ARCHITECTUREPlant Sciences;
 Abstract: Little is known about how the photosynthetic machinery is arranged in time and space during the biogenesis of thylakoid membranes. Using in situ cryo-electron tomography to image the three-dimensional architecture of the cyanobacterium Synechocystis, we observed that the tips of multiple thylakoids merge to form a substructure called the 'convergence membrane'. This high-curvature membrane comes into close contact with the plasma membrane at discrete sites. We generated subtomogram averages of 7OS ribosomes and array-forming phycobilisomes, then mapped these structures onto the native membrane architecture as markers for protein synthesis and photosynthesis, respectively. This molecular localization identified two distinct biogenic regions in the thylakoid network: thylakoids facing the cytosolic interior of the cell that were associated with both marker complexes, and convergence membranes that were decorated by ribosomes but not phycobilisomes. We propose that the convergence membranes perform a specialized biogenic function, coupling the synthesis of thylakoid proteins with the integration of cofactors from the plasma membrane and the periplasmic space.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000471019700020
DOI: 10.1038/s41477-019-0399-7
 Degree: -

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Title: Nature Plants
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 5 (4) Sequence Number: - Start / End Page: 436 - 446 Identifier: ISSN: 2055-0278
CoNE: https://pure.mpg.de/cone/journals/resource/2055-0278