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  Patch pipettes used for loading small cells with fluorescent indicator dyes

Neher, E., & Almers, W. (1987). Patch pipettes used for loading small cells with fluorescent indicator dyes. In I. Atwater, E. Rojas, & B. Soria (Eds.), Biophysics of the Pankreatic ß-Cells (pp. 1-5). Boston MA: Springer.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0003-EA8D-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-881C-C
Genre: Book Chapter

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978-1-4684-5314-0_1.pdf (Publisher version), 431KB
 
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 Creators:
Neher, E.1, Author           
Almers, W.1, Author           
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1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: In modern cell physiology, much important information has come from the intracellular application of membrane-impermeant substances, such as ion indicator dyes, second messengers, regulatory proteins, antibodies and messenger RNA. Such substances have been either injected into cells through microelectrodes or loaded into lipid vesicles and erythrocyte ghosts that were later allowed to fuse with the cell membrane. We have monitored cytoplasmic Ca2+ in mast cells with intracellularly applied fura-2, a new Ca-indicator dye.

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Language(s): eng - English
 Dates: 1987
 Publication Status: Issued
 Pages: -
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 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4684-5314-0_1
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Title: Biophysics of the Pankreatic ß-Cells
Source Genre: Book
 Creator(s):
Atwater, I., Editor
Rojas, E., Editor
Soria, B., Editor
Affiliations:
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Publ. Info: Boston MA : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 5 Identifier: -

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Title: Advances in Experimental Medicine and Biology
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Pages: - Volume / Issue: 211 Sequence Number: - Start / End Page: - Identifier: -