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  An unbiased approach elucidates variation in (S)-(+)-linalool, a context-specific mediator of a tri-trophic interaction in wild tobacco

He, J., Fandino, R. A., Halitschke, R., Luck, K., Köllner, T. G., Murdock, M. H., et al. (2019). An unbiased approach elucidates variation in (S)-(+)-linalool, a context-specific mediator of a tri-trophic interaction in wild tobacco. Proceedings of the National Academy of Sciences of the United States of America, 116(29), 14651-14660. doi:10.1073/pnas.1818585116.

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 Creators:
He, Jun1, Author           
Fandino, Richard A.2, Author           
Halitschke, Rayko1, Author           
Luck, Katrin3, Author           
Köllner, Tobias G.3, Author           
Murdock, Mark H.1, Author           
Ray, Rishav1, 4, Author           
Gase, Klaus1, Author           
Knaden, Markus5, Author           
Baldwin, Ian Thomas1, Author           
Schuman, Meredith C.1, Author           
Affiliations:
1Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              
2Research Group Dr. M. Knaden, Insect Behavior, Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421913              
3Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
4IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
5Research Group Dr. M. Knaden, Insect Behavior, Department of Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421913              

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 Abstract: Plant volatile organic compounds (VOCs) mediate many interactions, and the function of common VOCs is especially likely to depend on
ecological context. We used a genetic mapping population of wild
tobacco, Nicotiana attenuata, originating from a cross of 2 natural
accessions from Arizona and Utah, separated by the Grand Canyon,
to dissect genetic variation controlling VOCs. Herbivory-induced leaf
terpenoid emissions varied substantially, while green leaf volatile
emissions were similar. In a field experiment, only emissions of linalool,
a common VOC, correlated significantly with predation of the
herbivore Manduca sexta by native predators. Using quantitative
trait locus mapping and genome mining,we identified an (S)-(+)-linalool
synthase (NaLIS). Genome resequencing, gene cloning, and activity
assays revealed that the presence/absence of a 766-bp
sequence in NaLIS underlies the variation of linalool emissions in
26 natural accessions. We manipulated linalool emissions and composition
by ectopically expressing linalool synthases for both enantiomers,
(S)-(+)- and (R)-(−)-linalool, reported to oppositely affect M.
sexta oviposition, in the Arizona and Utah accessions.We used these
lines to test ovipositingmoths in increasingly complex environments.
The enantiomers had opposite effects on oviposition preference, but
themagnitude of the effect depended strongly both on plant genetic
background, and complexity of the bioassay environment. Our study
reveals that the emission of linalool, a common VOC, differs by
orders-of-magnitude among geographically interspersed conspecific
plants due to allelic variation in a linalool synthase, and that the
response of a specialist herbivore to linalool depends on enantiomer, plant genotype, and environmental complexity.

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 Dates: 20192019-07-012019-07-16
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: ITB609
DOI: 10.1073/pnas.1818585116
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 116 (29) Sequence Number: - Start / End Page: 14651 - 14660 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230