English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Spontaneously arising streptococcus mutans variants with reduced susceptibility to chlorhexidine display genetic defects and diminished fitness

Kaspar, J. R., Godwin, M. J., Velsko, I. M., Richards, V. P., & Burne, R. A. (2019). Spontaneously arising streptococcus mutans variants with reduced susceptibility to chlorhexidine display genetic defects and diminished fitness. Antimicrobial Agents and Chemotherapy, 63(7): e00161-19, pp. 1-17. doi:10.1128/AAC.00161-19.

Item is

Files

show Files
hide Files
:
shh2284.pdf (Publisher version), 6MB
 
File Permalink:
-
Name:
shh2284.pdf
Description:
-
OA-Status:
Visibility:
Private
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
Table S1-S4, Figure S1-S7 (Supplementary material)
Description:
pdf. - only available in institutes network. - (last seen: Dec. 2021)
OA-Status:

Creators

show
hide
 Creators:
Kaspar, Justin R., Author
Godwin, Matthew J., Author
Velsko, Irina Marie1, Author           
Richards, Vincent P., Author
Burne, Robert A., Author
Affiliations:
1Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              

Content

show
hide
Free keywords: Antimicrobial tolerance, Antimicrobials, Biofilms, Chlorhexidine, Dental caries,Microbial antagonism, Oral microbiome, Streptococcus mutans, Stress response
 Abstract: Chlorhexidine (CHX) has been used to control dental caries caused by acid-tolerant bacteria such as Streptococcus mutans since the 1970s. Repeat CHX exposure for other bacterial species results in the development of variants with reduced susceptibility that also become more resistant to other antimicrobials. It has not been tested if such variants arise when streptococci are exposed to CHX. Here, we passaged S. mutans in increasing concentrations of CHX and isolated spontaneously arising reduced susceptibility variants (RSVs) from separate lineages that have MICs that are up to 3-fold greater than the parental strain. The RSVs have increased growth rates at neutral pH and under acidic conditions in the presence of CHX but accumulate less biomass in biofilms. RSVs display higher MICs for daptomycin and clindamycin but increased sensitivity to dental-relevant antimicrobials triclosan and sodium fluoride. Plate-based assays for competition with health-associated oral streptococci revealed decreased bacteriocin production by the RSVs, increased sensitivity to hydrogen peroxide, and diminished competitive fitness in a human-derived ex vivo biofilm consortium. Whole-genome sequencing identified common single nucleotide polymorphisms (SNPs) within a diacylglycerol kinase homolog and a glycolipid synthesis enzyme, which could alter the accumulation of lipoteichoic acids and other envelope constituents, as well as a variety of mutations in other genes. Collectively, these findings confirm that S. mutans and likely other streptococci can develop tolerance to CHX but that increased tolerance comes at a fitness cost, such that CHX-induced variants that spontaneously arise in the human oral cavity may not persist. Copyright © 2019 American Society for Microbiology. All Rights Reserved.

Details

show
hide
Language(s): eng - English
 Dates: 2019-04-292019-07
 Publication Status: Issued
 Pages: 17
 Publishing info: -
 Table of Contents: Results and discussion
- Selection for variants with reduced susceptibility to chlorhexidine.
- Attributes of the RSV3s
- RSV3s have reduced susceptibility to other antimicrobials.
- RSV3s are less competitive against commensal oral streptococci.
- Whole-genome sequencing reveals mutations in RSVs
- Summary.
Material and methods
 Rev. Type: Peer
 Identifiers: DOI: 10.1128/AAC.00161-19
Other: shh2284
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Antimicrobial Agents and Chemotherapy
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 63 (7) Sequence Number: e00161-19 Start / End Page: 1 - 17 Identifier: ISSN: 0066-4804
CoNE: https://pure.mpg.de/cone/journals/resource/954925458050