English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Absolute quantification of cohesin, CTCF and their regulators in human cells.

Holzmann, J., Politi, A., Nagasaka, K., Hantsche-Grininger, M., Walther, N., Koch, B., et al. (2019). Absolute quantification of cohesin, CTCF and their regulators in human cells. eLife, 8: e46269. doi:10.7554/eLife.46269.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-3ED0-C Version Permalink: http://hdl.handle.net/21.11116/0000-0004-3ED6-6
Genre: Journal Article

Files

show Files
hide Files
:
3134900.pdf (Publisher version), 9MB
Name:
3134900.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
3134900_Suppl_1.htm (Supplementary material), 17KB
Name:
3134900_Suppl_1.htm
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/xhtml+xml / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3134900_Suppl_2.htm (Supplementary material), 15KB
Name:
3134900_Suppl_2.htm
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/xhtml+xml / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3134900_Suppl_3.pdf (Supplementary material), 321KB
Name:
3134900_Suppl_3.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Holzmann, J., Author
Politi, A.1, Author              
Nagasaka, K., Author
Hantsche-Grininger, M., Author
Walther, N., Author
Koch, B., Author
Fuchs, J., Author
Dürnberger, G., Author
Tang, W., Author
Ladurner, R., Author
Stocsits, R. R., Author
Busslinger, G. A., Author
Novak, B., Author
Mechtler, K., Author
Davidson, I. F., Author
Ellenberg, J., Author
Peters, J. M., Author
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

Content

show
hide
Free keywords: -
 Abstract: The organisation of mammalian genomes into loops and topologically associating domains (TADs) contributes to chromatin structure, gene expression and recombination. TADs and many loops are formed by cohesin and positioned by CTCF. In proliferating cells, cohesin also mediates sister chromatid cohesion, which is essential for chromosome segregation. Current models of chromatin folding and cohesion are based on assumptions of how many cohesin and CTCF molecules organise the genome. Here we have measured absolute copy numbers and dynamics of cohesin, CTCF, NIPBL, WAPL and sororin by mass spectrometry, fluorescence-correlation spectroscopy and fluorescence recovery after photobleaching in HeLa cells. In G1-phase, there are similar to 250,000 nuclear cohesin complexes, of which similar to 160,000 are chromatin-bound. Comparison with chromatin immunoprecipitation-sequencing data implies that some genomic cohesin and CTCF enrichment sites are unoccupied in single cells at any one time. We discuss the implications of these findings for how cohesin can contribute to genome organisation and cohesion.

Details

show
hide
Language(s): eng - English
 Dates: 2019-06-17
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.46269
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: eLife
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 31 Volume / Issue: 8 Sequence Number: e46269 Start / End Page: - Identifier: -