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  Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales

Schaper, S., Yau, H., Krol, E., Skotnicka, D., Heimerl, T., Gray, J., et al. (2018). Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales. PLOS GENETICS, 14(8): e1007594. doi:10.1371/journal.pgen.1007594.

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Schaper, S., Author
Yau, H., Author
Krol, E., Author
Skotnicka, D.1, Author           
Heimerl, T., Author
Gray, J., Author
Kaever, V., Author
Sogaard-Andersen, L1, Author           
Vollmer, W., Author
Becker, A., Author
Affiliations:
1Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, Karl-von-Frisch-Strasse 10, D-35043 Marburg, DE, ou_3266305              

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 Abstract: Members of the Rhizobiales (class of α-proteobacteria) display zonal peptidoglycan cell wall growth at one cell pole, contrasting with the dispersed mode of cell wall growth along the sidewalls of many other rod-shaped bacteria. Here we show that the seven-transmembrane receptor (7TMR) protein RgsP (SMc00074), together with the putative membrane-anchored peptidoglycan metallopeptidase RgsM (SMc02432), have key roles in unipolar peptidoglycan formation during growth and at mid-cell during cell division in Sinorhizobium meliloti. RgsP is composed of a periplasmic globular 7TMR-DISMED2 domain, a membrane-spanning region, and cytoplasmic PAS, GGDEF and EAL domains. The EAL domain confers phosphodiesterase activity towards the second messenger cyclic di-GMP, a key regulatory player in the transition between bacterial lifestyles. RgsP and RgsM localize to sites of zonal cell wall synthesis at the new cell pole and cell divison site, suggesting a role in cell wall biogenesis. The two proteins are essential for cell wall biogenesis and cell growth. Cells depleted of RgsP or RgsM had an altered muropeptide composition and RgsM binds to peptidoglycan. RgsP and RgsM orthologs are functional when interchanged between α-rhizobial species pointing to a conserved mechanism for cell wall biogenesis/remodeling within the Rhizobiales. Overall, our findings suggest that RgsP and RgsM contribute to the regulation of unipolar cell wall biogenesis in α-rhizobia.

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 Dates: 2018-08
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: eDoc: 747925
ISI: 000443389100043
DOI: 10.1371/journal.pgen.1007594
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Title: PLOS GENETICS
Source Genre: Journal
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Pages: - Volume / Issue: 14 (8) Sequence Number: e1007594 Start / End Page: - Identifier: ISSN: 1553-7404