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  Environmental microbial factors determine the pattern of inflammatory lesions in a murine model of Crohn’s Disease–Like inflammation

Stolzer, I., Kaden-Volynets, V., Ruder, B., Letizia, M., Bittel, M., Rausch, P., et al. (2019). Environmental microbial factors determine the pattern of inflammatory lesions in a murine model of Crohn’s Disease–Like inflammation. Inflammatory Bowel Diseases, 00(00): izz142. doi:10.1093/ibd/izz142.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-495E-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-495F-1
Genre: Journal Article

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 Creators:
Stolzer, Iris, Author
Kaden-Volynets, Valentina, Author
Ruder, Barbara, Author
Letizia, Marilena, Author
Bittel, Miriam, Author
Rausch, Philipp1, Author              
Basic, Marijana, Author
Bleich, André, Author
Baines, John F.1, Author              
Neurath, Markus F, Author
Wirtz, Stefan, Author
Weidinger, Carl, Author
Bischoff, Stephan C, Author
Becker, Christoph, Author
Günther, Claudia, Author
Affiliations:
1Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445638              

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Free keywords: Crohn’s disease, Paneth cells, ileitis, colitis, microflora, cell death
 Abstract: Crohn’s disease (CD) patients can be grouped into patients suffering from ileitis, ileocolitis, jejunoileitis, and colitis. The pathophysiological mechanism underlying this regional inflammation is still unknown. Although most murine models of inflammatory bowel disease (IBD) develop inflammation in the colon, there is an unmet need for novel models that recapitulate the spontaneous and fluctuating nature of inflammation as seen in CD. Recently, mice with an intestinal epithelial cell–specific deletion for Caspase-8 (Casp8ΔIEC mice), which are characterized by cell death–driven ileitis and disrupted Paneth cell homeostasis, have been identified as a novel model of CD-like ileitis. Here we uncovered that genetic susceptibility alone is sufficient to drive ileitis in Casp8ΔIEC mice. In sharp contrast, environmental factors, such as a disease-relevant microbial flora, determine colonic inflammation. Accordingly, depending on the microbial environment, isogenic Casp8ΔIEC mice either exclusively developed ileitis or suffered from pathologies in several parts of the gastrointestinal tract. Colitis in these mice was characterized by massive epithelial cell death, leading to spread of commensal gut microbes to the extra-intestinal space and hence an aberrant activation of the systemic immunity. We further uncovered that Casp8ΔIEC mice show qualitative and quantitative changes in the intestinal microbiome associated with an altered mucosal and systemic immune response. In summary, we identified that inflammation in this murine model of CD-like inflammation is characterized by an immune reaction, presumably directed against a disease-relevant microbiota in a genetically susceptible host, with impaired mucosal barrier function and bacterial clearance at the epithelial interface.

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Language(s): eng - English
 Dates: 2019-01-072019-07-052019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1093/ibd/izz142
 Degree: -

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Title: Inflammatory Bowel Diseases
  Other : Inflamm. Bowel Dis.
Source Genre: Journal
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Affiliations:
Publ. Info: New York, NY : Raven Press
Pages: - Volume / Issue: 00 (00) Sequence Number: izz142 Start / End Page: - Identifier: ISSN: 1078-0998
CoNE: https://pure.mpg.de/cone/journals/resource/954925606820