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  Miniaturized carbon dioxide sensor designed for measurements within plant leaves

Hanstein, S., de Beer, D., & Felle, H. H. (2001). Miniaturized carbon dioxide sensor designed for measurements within plant leaves. Sensors and Actuators b-Chemical, 81(1), 107-114.

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De_Beer_2001.pdf (Publisher version), 186KB
 
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 Creators:
Hanstein, Stefan, Author
de Beer, Dirk1, Author           
Felle, Hubert H., Author
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1Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481711              

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 Abstract: To permit CO2 measurements behind stomatal pores of plant leaves a potentiometric CO2-biosensor was built with a tip diameter of 2 μm. The miniaturised sensor assembly consists of a H+ carrier-based pH-microelectrode concentrically arranged within a sheathing micropipette. The tip of the latter is filled with carbonate buffer, the pH of which quickly responds to CO2 concentration changes due to incorporated carbonic anhydrase. The CO2-microsensor shows a linear response to CO2 in the physiologically relevant concentration range of 50–800 ppm (5.1–81.1 Pa). The 90% response time varied from sensor to sensor between 18 and 63 s (mean±S.E., 42±14 s; n=6). Sensor calibration and leaf experiments were performed in an open-flow tube-like minicuvette, allowing tangential air flow along the leaf surface with controlled gas mixtures and flow rates of choice. At 800 ppm external CO2 concentration, CO2 within the leaf was close to the external CO2 concentration when stomatal pores were wide open. However, the concentration dropped to 350 ppm during stomatal closure due to CO2 consumption by photosynthesis demonstrating distinct CO2-sensing of internal leaf CO2. Following ‘light-off’ internal CO2 rapidly rose close to 700 ppm, a response which was completely reversed by ‘light-on’. We conclude that this sensor is a suitable tool for CO2 monitoring in places too small to be accessible to conventional tools.

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Language(s): eng - English
 Dates: 2001-12-15
 Publication Status: Issued
 Pages: 8
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Title: Sensors and Actuators b-Chemical
  Other : Sens. Actuator B-Chem.
Source Genre: Journal
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Publ. Info: Lausanne, Switzerland : Elsevier
Pages: 8 Volume / Issue: 81 (1) Sequence Number: - Start / End Page: 107 - 114 Identifier: ISSN: 0925-4005
CoNE: https://pure.mpg.de/cone/journals/resource/954928549300