English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  NASC-seq monitors RNA synthesis in single cells.

Hendriks, G. J., Jung, L. A., Larsson, A. J. M., Lidschreiber, M., Forsman, O. A., Lidschreiber, K., et al. (2019). NASC-seq monitors RNA synthesis in single cells. Nature Communications, 10(1): 3138. doi:10.1038/s41467-019-11028-9.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-4D34-C Version Permalink: http://hdl.handle.net/21.11116/0000-0004-4D3A-6
Genre: Journal Article

Files

show Files
hide Files
:
3143549.pdf (Publisher version), 3MB
Name:
3143549.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
3143549_Suppl_1.pdf (Supplementary material), 2MB
Name:
3143549_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3143549_Suppl_2.pdf (Supplementary material), 69KB
Name:
3143549_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3143549_Suppl_3.pdf (Supplementary material), 800KB
Name:
3143549_Suppl_3.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Hendriks, G. J., Author
Jung, L. A., Author
Larsson, A. J. M., Author
Lidschreiber, M.1, Author              
Forsman, O. A., Author
Lidschreiber, K.1, Author              
Cramer, P.1, Author              
Sandberg, R., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: -
 Abstract: Sequencing of newly synthesised RNA can monitor transcriptional dynamics with great sensitivity and high temporal resolution, but is currently restricted to populations of cells. Here, we develop new transcriptome alkylation-dependent single-cell RNA sequencing (NASC-seq), to monitor newly synthesised and pre-existing RNA simultaneously in single cells. We validate the method on pre-labelled RNA, and by demonstrating that more newly synthesised RNA was detected for genes with known high mRNA turnover. Monitoring RNA synthesis during Jurkat T-cell activation with NASC-seq reveals both rapidly up- and down-regulated genes, and that induced genes are almost exclusively detected as newly transcribed. Moreover, the newly synthesised and pre-existing transcriptomes after T-cell activation are distinct, confirming that NASC-seq simultaneously measures gene expression corresponding to two time points in single cells. Altogether, NASC-seq enables precise temporal monitoring of RNA synthesis at single-cell resolution during homoeostasis, perturbation responses and cellular differentiation.

Details

show
hide
Language(s): eng - English
 Dates: 2019-07-17
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-019-11028-9
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 9 Volume / Issue: 10 (1) Sequence Number: 3138 Start / End Page: - Identifier: -