English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots

Breus, V., Pietuch, A., Tarantola, M., Basche, T., & Janshoff, A. (2015). The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots. Beilstein Journal of Nanotechnology, 6, 281-292. doi:10.3762/bjnano.6.26.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-4D41-D Version Permalink: http://hdl.handle.net/21.11116/0000-0004-57DF-0
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Breus, V., Author
Pietuch, A., Author
Tarantola, Marco1, Author              
Basche, T., Author
Janshoff, A., Author
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, DE, ou_2063287              

Content

show
hide
Free keywords: biocompatibility; CdSe/ZnS; cytotoxicity; ECIS; quantum dots; single-particle tracking
 Abstract: In this work, cytotoxicity and cellular impedance response was compared for CdSe/ZnS core/shell quantum dots (QDs) with positively charged cysteamine–QDs, negatively charged dihydrolipoic acid–QDs and zwitterionic D-penicillamine–QDs exposed to canine kidney MDCKII cells. Pretreatment of cells with pharmacological inhibitors suggested that the uptake of nanoparticles was largely due to receptor-independent pathways or spontaneous entry for carboxylated and zwitterionic QDs, while for amine-functionalized particles involvement of cholesterol-enriched membrane domains is conceivable. Cysteamine–QDs were found to be the least cytotoxic, while D-penicillamine–QDs reduced the mitochondrial activity of MDCKII by 20–25%. Although the cell vitality appeared unaffected (assessed from the changes in mitochondrial activity using a classical MTS assay after 24 h of exposure), the binding of QDs to the cellular interior and their movement across cytoskeletal filaments (captured and characterized by single-particle tracking), was shown to compromise the integrity of the cytoskeletal and plasma membrane dynamics, as evidenced by electric cell–substrate impedance sensing.

Details

show
hide
Language(s): eng - English
 Dates: 2015-01-26
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.3762/bjnano.6.26
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Beilstein Journal of Nanotechnology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 6 Sequence Number: - Start / End Page: 281 - 292 Identifier: -