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  External RBS/PIXE analysis for evaluating quantum dots internalization into HeLa cells.

Saito, M., Koike, S., Holm, K., Bregolin, F. L., & Hofsäss, H. (2019). External RBS/PIXE analysis for evaluating quantum dots internalization into HeLa cells. Nuclear Instruments and Methods in Physics Research B, 450, 173-178. doi:10.1016/j.nimb.2018.08.027.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-5237-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-523A-F
Genre: Journal Article

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 Creators:
Saito, M., Author
Koike, S.1, Author              
Holm, K., Author
Bregolin, F. L., Author
Hofsäss, H., Author
Affiliations:
1Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              

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Free keywords: External RBS; HeLa cells; Quantum dot; Internalization
 Abstract: We have developed an external RBS/PIXE setup at University of Gottingen. By using this setup, the analysis can be performed in atmospheric condition, which leads to realizing a quantitative analysis for volatile samples with depth information. We applied this technique for HeLa cells, internalized CdSe quantum dots (Q-dot). External RBS shows the depth difference of Q-dot depth distribution in cells, w or w/o the treatment with an endocytosis inhibitor, which agrees well with the result of confocal microscopy. The quantity of Q-dot is also evaluated by external PIXE, which coincides with the result of RBS and fluorescence measurement. In addition, the application of Si3N4 film as a medium for HeLa cells is presented.

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Language(s): eng - English
 Dates: 2019-07-01
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.nimb.2018.08.027
 Degree: -

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Title: Nuclear Instruments and Methods in Physics Research B
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 450 Sequence Number: - Start / End Page: 173 - 178 Identifier: -